Immobilization of Penicillin Acylase on Macroporous Adsorption Resin CLX1180 Carrier

doi:10.1002/adv.21717

	Immobilization of Penicillin Acylase on Macroporous Adsorption Resin CLX1180 Carrier
	Liu, Donglei ; Chen, Zhenbin 1; Long, Jiapeng ; Zhao, Yingyu ; Du, Xueyan
刊名	ADVANCES IN POLYMER TECHNOLOGY
	2018-04
卷号	37 期号:3 页码:753-760
关键词	Diffusion-controlled reaction Immobilization of penicillin acylase Nucleophilic substitution
ISSN号	0730-6679
DOI	10.1002/adv.21717
英文摘要	This work reported a novel immobilization method of penicillin acylase (PA) using nucleophilic substitution reaction between benzyl chloride on macroporous adsorption resin (MAR) and amino groups on PA. Nucleophilic substitution reaction of benzyl chloride was reported for the first time in enzyme immobilization. The work was conducted as follows: Firstly, an apolar MAR, LX1180, was chemically modified, that is, chloromethylated, to prepare benzyl chloride LX1180 (CLX1180). Then, CLX1180 was used as a support to immobilize PA through nucleophilic substitution. The influence of reaction conditions, such as immobilization time, temperature, pH, and the amount of PA, on the loading ratio and catalytic activity were investigated and optimized. Besides, this study indicated that the immobilization reaction was a diffusion-controlled reaction. Finally, the structure of immobilized PA was characterized using FTIR, and the stability for catalyst activity was explored by investigating the reusability of the sample, and the result indicated that PA was immobilized on the surface of CLX1180 successfully and presented an excellent stability of catalyst activity. It proposed a simple and promising way to immobilize PA for enzyme catalysis.
资助项目	Lanzhou University of Technology[SB01200806] ; Gansu Provincial Department of Education[1001ZSB002] ; Natural Science Foundation of China[51563015]
WOS研究方向	Engineering ; Polymer Science
语种	英语
出版者	WILEY
WOS记录号	WOS:000430100600013
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/32707]
专题	材料科学与工程学院省部共建有色金属先进加工与再利用国家重点实验室
通讯作者	Chen, Zhenbin
作者单位	1.Lanzhou Univ Technol, State Key Lab Gansu Adv Nonferrous Met Mat, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Univ Technol, Sch Mat Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Liu, Donglei,Chen, Zhenbin,Long, Jiapeng,et al. Immobilization of Penicillin Acylase on Macroporous Adsorption Resin CLX1180 Carrier[J]. ADVANCES IN POLYMER TECHNOLOGY,2018,37(3):753-760.
APA	Liu, Donglei,Chen, Zhenbin,Long, Jiapeng,Zhao, Yingyu,&Du, Xueyan.(2018).Immobilization of Penicillin Acylase on Macroporous Adsorption Resin CLX1180 Carrier.ADVANCES IN POLYMER TECHNOLOGY,37(3),753-760.
MLA	Liu, Donglei,et al."Immobilization of Penicillin Acylase on Macroporous Adsorption Resin CLX1180 Carrier".ADVANCES IN POLYMER TECHNOLOGY 37.3(2018):753-760.