Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum

doi:10.1016/j.fbio.2022.101664

CORC > 兰州理工大学 > 兰州理工大学

	Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum
	Ma, Wenjin 1,2,5; Zhou, Yanbing 3; Lou, Wenjin 4; Wang, Bo 3; Li, Bing 3; Liu, Xiaofen 5; Yang, Jiajun 5; Yang, Bo 5; Liu, Jianfei 1,2; Di, Duolong 1,2
刊名	FOOD BIOSCIENCE
	2022-06-01
卷号	47
关键词	Lycium barbarum polysaccharide Lung cancer cell Apoptosis Cell cycle Structural analysis
ISSN号	2212-4292
DOI	10.1016/j.fbio.2022.101664
英文摘要	The water-soluble polysaccharide LBP-1 was isolated and characterized from Lycium barbarum L. LBP-1 was mainly composed of arabinose, galactose, glucose, xylose, mannose at a molar ratio of 37.53: 28.08: 14.72: 7.83: 4.50, respectively. Based on nuclear magnetic resonance (NMR) and methylation analysis, the backbone of LBP-1 was speculated in the alpha-L-Ara(1 ->[5-alpha-L-Ara(1 -> 3)-8-D-Galp-(1 ->]n -> 4)-alpha-D-Galp-(1[-> 5-alpha-L-Ara (1]n [-> 6)8-D-Galp-(1 -> 4)-8-D-Galp-(1 ->]n, and the side chains of LBP-1 were in the alpha-L-Ara(1 -> 3)-8-D-Galp-(1 -> 6 position. These results showed that LBP-1 inhibited the growth of cancer A549 cells through cell cycle arrest and apoptosis, with an IC50 value of 42.5 mu g/mL. In addition, LBP-1 altered the expression of Cyclin D1, Cyclin D3, and CDK 2, thus blocking the cell cycle in G0/G1 phase, reducing cell migration, and regulating the PI3K/Akt/ mTOR signaling pathway to induce apoptosis.
WOS研究方向	Food Science & Technology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000793051600002
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/158952]
专题	兰州理工大学
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, CAS Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China; 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China; 3.Inst Gansu Prov Light Ind Sci Res, Lanzhou 730000, Peoples R China; 4.Gansu Prov Acad Ind Med Res, Lanzhou 730000, Peoples R China; 5.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Ma, Wenjin,Zhou, Yanbing,Lou, Wenjin,et al. Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum[J]. FOOD BIOSCIENCE,2022,47.
APA	Ma, Wenjin.,Zhou, Yanbing.,Lou, Wenjin.,Wang, Bo.,Li, Bing.,...&Di, Duolong.(2022).Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum.FOOD BIOSCIENCE,47.
MLA	Ma, Wenjin,et al."Mechanism regulating the inhibition of lung cancer A549 cell proliferation and structural analysis of the polysaccharide Lycium barbarum".FOOD BIOSCIENCE 47(2022).