Protective effect of rhodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury

doi:10.4103/1673-5374.266920

CORC > 兰州理工大学 > 兰州理工大学

	Protective effect of rhodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury
	Ha, Xiao-Qin 1; Yang, Bo 2,4; Hou, Huai-Jing 2; Cai, Xiao-Ling 3; Xiong, Wan-Yuan 2; Wei, Xu-Pan 2
刊名	NEURAL REGENERATION RESEARCH
	2020-04-01
卷号	15 期号:4 页码:690-696
关键词	acute spinal cord injury adenovirus adenovirus gene IX bone marrow mesenchymal stem cells combined behavioral score scale HIF-1 alpha nerve regeneration nerve repair Rhodiola rosea Sry
ISSN号	1673-5374
DOI	10.4103/1673-5374.266920
英文摘要	Rhodioloside has been shown to protect cells from hypoxia injury, and bone marrow mesenchymal stem cells have a good effect on tissue repair. To study the effects of rhodioloside and bone marrow mesenchymal stem cells on spinal cord injury, a rat model of spinal cord injury was established using the Infinite Horizons method. After establishing the model, the rats were randomly divided into five groups. Rats in the control group were intragastrically injected with phosphate buffered saline (PBS) (5 mu L). PBS was injected at 6 equidistant points around 5 mm from the injury site and at a depth of 5 mm. Rats in the rhodioloside group were intragastrically injected with rhodioloside (5 g/kg) and intramuscularly injected with PBS. Rats in the mesenchymal stem cell (MSC) group were intramuscularly injected with PBS and intramuscularly with MSCs (8 x 10(6)/mL in a 50-mu L cell suspension). Rats in the Ad-HIF-MSC group were intragastrically injected with PBS and intramuscularly injected with HIF-1 adenovirus-infected MSCs. Rats in the rhodioloside + Ad-HIF-MSC group were intramuscularly injected with MSCs infected with the HIF-1 adenovirus and intragastrically injected with rhodioloside. One week after treatment, exercise recovery was evaluated with a modified combined behavioral score scale. Hematoxylin-eosin staining and Pischingert's methylene blue staining were used to detect any histological or pathological changes in spinal cord tissue. Levels of adenovirus IX and Sry mRNA were detected by real-time quantitative polymerase chain reaction and used to determine the number of adenovirus and mesenchymal stem cells that were transfected into the spinal cord. Immunohistochemical staining was applied to detect HIF-1 protein levels in the spinal cord. The results showed that: (1) compared with the other groups, the rhodioloside + Ad-HIF-MSC group exhibited the highest combined behavioral score (P < 0.05), the most recovered tissue, and the greatest number of neurons, as indicated by Pischingert's methylene blue staining. (2) Compared with the PBS group, HIF-1 protein expression was greater in the rhodioloside group (P < 0.05). (3) Compared with the Ad-HIF-MSC group, Sry mRNA levels were higher in the rhodioloside + Ad-HIF-MSC group (P < 0.05). These results confirm that rhodioloside combined with bone marrow mesenchymal stem cells can promote the recovery of spinal cord injury and activate the HIF-1 pathway to promote the survival of bone marrow mesenchymal stem cells and repair damaged neurons within spinal cord tissue. This experiment was approved by the Animal Ethics Committee of Gansu University of Traditional Chinese Medicine, China (approval No. 2015KYLL029) in June 2015.
WOS研究方向	Cell Biology ; Neurosciences & Neurology
语种	英语
出版者	WOLTERS KLUWER MEDKNOW PUBLICATIONS
WOS记录号	WOS:000503237500015
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/155218]
专题	兰州理工大学
作者单位	1.Lanzhou Univ, Hosp 2, Lanzhou, Gansu, Peoples R China; 2.Gansu Univ Tradit Chinese Med, Sch Clin Med, Lanzhou, Gansu, Peoples R China; 3.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China 4.Lanzhou Mil Area Command, Dept Clin Lab, Lanzhou Gen Hosp, Lanzhou, Gansu, Peoples R China;
推荐引用方式 GB/T 7714	Ha, Xiao-Qin,Yang, Bo,Hou, Huai-Jing,et al. Protective effect of rhodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury[J]. NEURAL REGENERATION RESEARCH,2020,15(4):690-696.
APA	Ha, Xiao-Qin,Yang, Bo,Hou, Huai-Jing,Cai, Xiao-Ling,Xiong, Wan-Yuan,&Wei, Xu-Pan.(2020).Protective effect of rhodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury.NEURAL REGENERATION RESEARCH,15(4),690-696.
MLA	Ha, Xiao-Qin,et al."Protective effect of rhodioloside and bone marrow mesenchymal stem cells infected with HIF-1-expressing adenovirus on acute spinal cord injury".NEURAL REGENERATION RESEARCH 15.4(2020):690-696.