Dynamic variations of microbial community structure in Myriophyllum aquaticum constructed wetlands in response to different NH4+-N concentrations

doi:10.1016/j.procbio.2020.02.028

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	Dynamic variations of microbial community structure in Myriophyllum aquaticum constructed wetlands in response to different NH4+-N concentrations
	Xu, Lina 2,3; Zhang, Bao 1,3; Peng, Xiawei 2; Zhang, Xupo 3; Sun, Bo 3; Sun, Haishu 3; Jiang, Cancan 3; Zhou, Sining 3; Zeng, Xiangui 4; Bai, Zhihui 3
刊名	Process Biochemistry
	2020-06-01
卷号	93 页码:55-62
关键词	Denitrification Genes Microorganisms Polymerase chain reaction Ammonium nitrogen Constructed wetlands Denitrifiers Microbial community structures Myriophyllum aquaticum
ISSN号	13595113
DOI	10.1016/j.procbio.2020.02.028
英文摘要	Constructed wetlands (CWs) have received increasing attentions for their N removal performances, especially regarding NH4+-N. Different influent NH4+-N concentration may influence N removal efficiency in practice, while the effects of different NH4+-N concentrations on microorganisms removing N in CWs are poorly understood. In this study, surface flow CWs planted with Myriophyllum (M). aquaticum were established to investigate the influences of different NH4+-N concentrations on the composition, structure, and interactions of microbial community. Our findings suggested 105 mg/L NH4+-N CWs achieved highest N removal rate, removing 89.30 % NH4+-N and 92.34 % TN from the influent. The results of real-time quantitative polymerase chain reactions (qPCR) indicated abundances of nitrifying genes (nxrA) and denitrifying genes (narG, nirS, nirK, and nosZ) were increased by increasing NH4+-N concentrations, and the strongest effects were observed in narG (8-fold) and nosZ genes (11-fold). Different NH4+-N concentrations was proved to alter composition and structure of microbial communities via high-throughput sequencing, e.g. denitrifiers including Brevendomonas.sp, Dokdonella.sp and Rhodococcus.sp were enriched obviously with increasing NH4+-N concentrations. In addition, network showed interactions among microbial populations and positive interactions were dramatically shifted and enhanced by increasing NH4+-N concentrations. © 2020
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
语种	英语
出版者	Elsevier Ltd
WOS记录号	WOS:000531102600007
内容类型	期刊论文
源URL	[http://ir.lut.edu.cn/handle/2XXMBERH/115825]
专题	兰州理工大学土木工程学院
作者单位	1.College of Civil Engineering, Lanzhou University of Technology, Lanzhou; 730050, China; 2.College of Biological Sciences and Technology, Beijing Forestry University, Beijing; 100083, China; 3.Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing; 100085, China; 4.Shenzhen DiDa Water Engineering Limited Company, Shenzhen; 518116, China
推荐引用方式 GB/T 7714	Xu, Lina,Zhang, Bao,Peng, Xiawei,et al. Dynamic variations of microbial community structure in Myriophyllum aquaticum constructed wetlands in response to different NH4+-N concentrations[J]. Process Biochemistry,2020,93:55-62.
APA	Xu, Lina.,Zhang, Bao.,Peng, Xiawei.,Zhang, Xupo.,Sun, Bo.,...&Zhuang, Xuliang.(2020).Dynamic variations of microbial community structure in Myriophyllum aquaticum constructed wetlands in response to different NH4+-N concentrations.Process Biochemistry,93,55-62.
MLA	Xu, Lina,et al."Dynamic variations of microbial community structure in Myriophyllum aquaticum constructed wetlands in response to different NH4+-N concentrations".Process Biochemistry 93(2020):55-62.