Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells

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	Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells
	Wang, Peng 1,2; Wang, Yunshan 1,2; Su, Zhiguo 1,2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2012-02-01
卷号	166 期号:4 页码:974-986
关键词	Propionibacterium freudenreichii Propionic acid ISPR External direct mode Fed-batch fermentation
ISSN号	0273-2289
通讯作者	Wang, YS
英文摘要	The recovery of an inhibiting product from a bioreactor soon after its formation is an important issue in industrial bioprocess development. In the present study, the potential of the anion exchanger-based in situ product recovery (ISPR) technique for the biocatalytic production of propionic acid was discussed. The focus of the current work was the selection of a suitable configuration of metabolically active cells for application in propionic acid production. Accumulation of propionic acid in fermentation broth caused feedback inhibition of the growth and biotransformation activity of Propionibacterium freudenreichii CICC 10019. Relevant product inhibition kinetics was discussed, and the results showed that keeping the aqueous propionic acid concentration below 10.02 g L-1 was an essential prerequisite for ISPR process. A batch study, in which three ISPR configuration mode designs were compared, was conducted. The comparison indicated that employing an external direct mode had significant advantages over other modes in terms of increased productivity and product yield, with a corresponding decrease in the number of downstream processing steps, as well as in substrate consumption. The fed-batch culture using an external direct mode for the continuous accumulation of propionic acid resulted in a cumulative propionic acid concentration of 62.5 g L-1, with a corresponding product yield of 0.78 g propionic acid/g glucose.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
研究领域[WOS]	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
关键词[WOS]	LACTIC-ACID ; BATCH FERMENTATION ; ACIDIPROPIONICI ; BIOREACTOR ; REMOVAL ; EXTRACTION ; MEMBRANE
收录类别	SCI
语种	英语
WOS记录号	WOS:000300296700014
公开日期	2013-10-26
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/4296]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Wang, Peng,Wang, Yunshan,Su, Zhiguo. Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2012,166(4):974-986.
APA	Wang, Peng,Wang, Yunshan,&Su, Zhiguo.(2012).Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,166(4),974-986.
MLA	Wang, Peng,et al."Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 166.4(2012):974-986.