Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG

doi:10.4238/2013.April.25.11

CORC > 兰州理工大学 > 兰州理工大学

	Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG
	Ma, J.1; Wang, M. G.1; Mao, A. H.2; Zeng, J. Y.3; Liu, Y. Q.2; Wang, X. Q.2; Ma, J.2; Tian, Y. J.4; Ma, N.4; Yang, N.3
刊名	GENETICS AND MOLECULAR RESEARCH
	2013
卷号	12 期号:2 页码:1399-1410
关键词	Target replacement DNA aptamer Fc region of mouse IgG SELEX
ISSN号	1676-5680
DOI	10.4238/2013.April.25.11
英文摘要	Aptamers that recognize the IgG Fc region are of great interest because of their wide application as an immunology probing tool, for diagnostics, and as affinity agents for antibody purification. We developed a target replacement strategy as a modification of conventional Systematic Evolution of Ligands by EXponential enrichment (SELEX) in order to efficiently select and identify novel DNA aptamers against the Fc region of mouse IgG. In this new approach, multiple IgG subclasses (IgG1, IgG2a, mouse IgG Fc, and anti-HBs IgG) were sequentially used to select aptamers in one continuous SELEX. After 8 rounds of selection, the aptamers were analyzed using dot blot and an electrophoretic mobility shift assay, which showed universal binding capability to different IgG subclasses. Secondary structure analysis of the aptamers indicated that the stem-loop structure of the aptamers play an important role in binding to the common site in different mouse IgG subclasses. This demonstrated the feasibility of using multiple target replacement SELEX for the selection of aptamers. This target replacement strategy is also expected to be useful for selecting aptamers that bind common regions of molecules other than antibodies.
资助项目	Fund of Innovative Technology for Small or Medium-Sized Enterprises in China[06C26226200591]
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	FUNPEC-EDITORA
WOS记录号	WOS:000320030100054
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/34795]
专题	兰州理工大学
通讯作者	Liao, S. Q.
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou, Gansu, Peoples R China 2.Gansu Acad Med Sci, Lanzhou, Gansu, Peoples R China 3.Northwest Normal Univ, Coll Life Sci, Lanzhou, Gansu, Peoples R China 4.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Ma, J.,Wang, M. G.,Mao, A. H.,et al. Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG[J]. GENETICS AND MOLECULAR RESEARCH,2013,12(2):1399-1410.
APA	Ma, J..,Wang, M. G..,Mao, A. H..,Zeng, J. Y..,Liu, Y. Q..,...&Liao, S. Q..(2013).Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG.GENETICS AND MOLECULAR RESEARCH,12(2),1399-1410.
MLA	Ma, J.,et al."Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG".GENETICS AND MOLECULAR RESEARCH 12.2(2013):1399-1410.