Pulsed electromagnetic fields prevented the decrease of bone formation in hindlimb-suspended rats by activating sAC/cAMP/PKA/CREB signaling pathway

doi:10.1002/bem.22150

CORC > 兰州理工大学 > 兰州理工大学

	Pulsed electromagnetic fields prevented the decrease of bone formation in hindlimb-suspended rats by activating sAC/cAMP/PKA/CREB signaling pathway
	Li, Wen-Yuan 1,2; Li, Xue-Yan 1; Tian, Yong-Hui 1; Chen, Xin-Ru 3; Zhou, Jian 2; Zhu, Bao-Ying 2; Xi, Hui-Rong 2; Gao, Yu-Hai 2; Xian, Cory J.4; Chen, Ke-Ming 2
刊名	BIOELECTROMAGNETICS
	2018-12
卷号	39 期号:8 页码:569-584
关键词	bone formation bone resorption hindlimb suspension microgravity pulsed electromagnetic fields
ISSN号	0197-8462
DOI	10.1002/bem.22150
英文摘要	Microgravity is one of the main threats to the health of astronauts. Pulsed electromagnetic fields (PEMFs) have been considered as one of the potential countermeasures for bone loss induced by space flight. However, the optimal therapeutic parameters of PEMFs have not been obtained and the action mechanism is still largely unknown. In this study, a set of optimal therapeutic parameters for PEMFs (50 Hz, 0.6 mT 50% duty cycle and 90 min/day) selected based on high-throughput screening with cultured osteoblasts was used to prevent bone loss in rats induced by hindlimb suspension, a commonly accepted animal model to simulate the space environment. It was found that hindlimb suspension for 4 weeks led to significant decreases in femoral and vertebral bone mineral density (BMD) and their maximal loads, severe deterioration in bone micro-structure, and decreases in levels of bone formation markers and increases in bone resorption markers. PEMF treatment prevented about 50% of the decreased BMD and maximal loads, preserved the microstructure of cancellous bone and thickness of cortical bone, and inhibited decreases in bone formation markers. Histological analyses revealed that PEMFs significantly alleviated the reduction in osteoblast number and inhibited the increase in adipocyte number in the bone marrow. PEMFs also blocked decreases in serum levels of parathyroid hormone and its downstream signal molecule cAMP, and maintained the phosphorylation levels of protein kinase A (PKA) and cAMP response element-binding protein (CREB). The expression level of soluble adenylyl cyclases (sAC) was also maintained. It therefore can be concluded that PEMFs partially prevented the bone loss induced by weightless environment by maintaining bone formation through signaling of the sAC/cAMP/PKA/CREB pathway. Bioelectromagnetics. 39:569-584, 2018. (c) 2018 Wiley Periodicals, Inc.
资助项目	NHMRC Australia Senior Research Fellowship[1042105]
WOS研究方向	Life Sciences & Biomedicine - Other Topics ; Biophysics
语种	英语
出版者	WILEY
WOS记录号	WOS:000450844400001
状态	已发表
内容类型	期刊论文
源URL	[http://119.78.100.223/handle/2XXMBERH/32320]
专题	兰州理工大学
通讯作者	Li, Xue-Yan; Chen, Ke-Ming
作者单位	1.Lanzhou Univ Technol, Sch Life Sci & Engn, Lanzhou 730050, Gansu, Peoples R China 2.Lanzhou Gen Hosp CPLA, Inst Orthopaed, Lanzhou, Gansu, Peoples R China 3.Northwest A&F Univ, Coll Life Sci, Yanglin, Peoples R China 4.Univ South Australia, Sch Pharm & Med Sci, Sansom Inst Hlth Res, Adelaide, SA, Australia
推荐引用方式 GB/T 7714	Li, Wen-Yuan,Li, Xue-Yan,Tian, Yong-Hui,et al. Pulsed electromagnetic fields prevented the decrease of bone formation in hindlimb-suspended rats by activating sAC/cAMP/PKA/CREB signaling pathway[J]. BIOELECTROMAGNETICS,2018,39(8):569-584.
APA	Li, Wen-Yuan.,Li, Xue-Yan.,Tian, Yong-Hui.,Chen, Xin-Ru.,Zhou, Jian.,...&Chen, Ke-Ming.(2018).Pulsed electromagnetic fields prevented the decrease of bone formation in hindlimb-suspended rats by activating sAC/cAMP/PKA/CREB signaling pathway.BIOELECTROMAGNETICS,39(8),569-584.
MLA	Li, Wen-Yuan,et al."Pulsed electromagnetic fields prevented the decrease of bone formation in hindlimb-suspended rats by activating sAC/cAMP/PKA/CREB signaling pathway".BIOELECTROMAGNETICS 39.8(2018):569-584.