Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

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	Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model
	Liu, Chen-Wei 1; Xiong, Feng 2; Jia, Hui-Zhen 1; Wang, Xu-Li 3; Cheng, Han 1; Sun, Yong-Hua 2; Zhang, Xian-Zheng 1; Zhuo, Ren-Xi 1; Feng, Jun 1
刊名	BIOMACROMOLECULES
	2013-02-01
卷号	14 期号:2 页码:358-366
关键词	DRUG-DELIVERY LIPOSOMAL DOXORUBICIN PHOTOTHERMAL THERAPY CARBON NANOTUBES CANCER-THERAPY IN-VITRO OXIDE NANOPARTICLES DISCOVERY CHEMISTRY
ISSN号	1525-7797
通讯作者	Sun, YH (reprint author), Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China.
中文摘要	In this paper, a facile strategy to develop graphene-based delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to self-assembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEG-polyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against He La cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.
英文摘要	In this paper, a facile strategy to develop graphene-based delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to self-assembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEG-polyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against He La cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
关键词[WOS]	DRUG-DELIVERY ; IN-VITRO ; LIPOSOMAL DOXORUBICIN ; PHOTOTHERMAL THERAPY ; CARBON NANOTUBES ; CANCER-THERAPY ; OXIDE ; TOXICITY ; WATER ; NANOPARTICLES
收录类别	SCI
资助信息	National Key Basic Research Program of China [2011CB606202, 2009CB930301]; National Natural Science Foundation of China [21174110]
语种	英语
WOS记录号	WOS:000314908500009
公开日期	2013-10-31
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19308]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Wuhan Univ, Dept Chem, Key Lab Biomed Polymers, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hyrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430071, Peoples R China 3.Univ Utah, Dept Pharmaceut & Pharmaceut Chem, Salt Lake City, UT 84108 USA
推荐引用方式 GB/T 7714	Liu, Chen-Wei,Xiong, Feng,Jia, Hui-Zhen,et al. Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model[J]. BIOMACROMOLECULES,2013,14(2):358-366.
APA	Liu, Chen-Wei.,Xiong, Feng.,Jia, Hui-Zhen.,Wang, Xu-Li.,Cheng, Han.,...&Feng, Jun.(2013).Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model.BIOMACROMOLECULES,14(2),358-366.
MLA	Liu, Chen-Wei,et al."Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model".BIOMACROMOLECULES 14.2(2013):358-366.