Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals
	Wei, Ting 1,2; Zhang, Chao 1,2; Xu, Xin 3; Hanna, Michelle 4; Zhang, Xiaohua 1; Wang, Yan 1; Dai, Heping 1; Xiao, Wei 3,4
刊名	BIOSENSORS & BIOELECTRONICS
	2013-06-15
卷号	44 页码:138-145
关键词	Genotoxin Reporter Biosensor Yeast Sensitivity
ISSN号	0956-5663
通讯作者	Dai, HP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
中文摘要	It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要	It has been well established that essentially all microbial mutagens are rodent carcinogens, yet current mutagen detection systems are limited by their detection sensitivity. Here we report the construction of a pair of hypersensitive biosensors by optimizing both reporters and the host strain. The resulting RNR3-yEGFP and HUG1-yEGFP reporters and the septuple yeast mutant in combination with the automated protocol not only remarkably enhance the detection sensitivity, but also allow a high throughput screen of environmental genotoxins. This system is deemed much more sensitive than similar yeast and bacterium-based tests for all selected chemicals examined in this study. (C) 2013 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]	PROMOTER-REPORTER CONSTRUCT ; GREEN FLUORESCENT PROTEIN ; INDUCED GENE-EXPRESSION ; DNA-DAMAGE ; SACCHAROMYCES-CEREVISIAE ; ESCHERICHIA-COLI ; TESTING SYSTEM ; ENHANCES SENSITIVITY ; CWP GENES ; ASSAY
收录类别	SCI
资助信息	Natural Science Foundation of China [21037004, 20977108]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ10]; Natural Sciences and Engineering Research Council of Canada [138338-2009]
语种	英语
WOS记录号	WOS:000317150700024
公开日期	2013-10-31
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19336]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China 4.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
推荐引用方式 GB/T 7714	Wei, Ting,Zhang, Chao,Xu, Xin,et al. Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals[J]. BIOSENSORS & BIOELECTRONICS,2013,44:138-145.
APA	Wei, Ting.,Zhang, Chao.,Xu, Xin.,Hanna, Michelle.,Zhang, Xiaohua.,...&Xiao, Wei.(2013).Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals.BIOSENSORS & BIOELECTRONICS,44,138-145.
MLA	Wei, Ting,et al."Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals".BIOSENSORS & BIOELECTRONICS 44(2013):138-145.