Toxicity of allelochemicals released by submerged macrophytes on phytoplankton

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	Toxicity of allelochemicals released by submerged macrophytes on phytoplankton
	Wang, J.; Zhu, J. Y.; Gao, Y. N.; Liu, B. Y.; Liu, S. P.; He, F.; Wu, Z. B.
刊名	ALLELOPATHY JOURNAL
	2013
卷号	31 期号:1 页码:199-209
关键词	Allelochemical cell volume chlorophyll a fluorescence esterase activity flow cytometry internal cell complexity membrane integrity phytoplankton
ISSN号	0971-4693
通讯作者	Liu, BY (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	The allelopathic mechanism of submerged macrophytes was investigated on the cyanobacterium Microcystis aeruginosa and the green alga Pseudokirchneriella subcapitata. The effect of the allelochemicals pyrogallic acid (PA), gallic acid (GA), (+)-catechin (CA), caffeic acid (CFA), protocatechuic acid (PCA), nonanoic acid (NA) and cis-9-octadecenoic acid (COA) was evaluated by flow cytometry on esterase activity, membrane integrity, chlorophyll a fluorescence, cell volume and internal cell complexity. The highest sensitivity was observed for esterase activity in both test species. NA damaged the cell membrane and severely altered the internal cell complexity of both species. PA changed the esterase activity of both species and significantly decreased the fluorescence of chlorophyll a in M. aeruginosa cells (P < 0.05). Both GA and PCA inhibited the fluorescence of chlorophyll a and caused morphological changes in M. aeruginosa (P < 0.05). CFA inhibited the esterase activity, fluorescence of chlorophyll a and cell volume in M. aeruginosa at 50 and 100 mg L-1 concentrations; In P. subcapitata, the fluorescence of chlorophyll a and cell volume was inhibited at 50 mg L-1 but was stimulated at 100 mg L-1. COA changed the esterase activity. CA at 100 mg L-1 slightly inhibited the fluorescence of chlorophyll a in M. aeruginosa and stimulated the esterase activity in P. subcapitata. The responses observed for each parameter investigated were highly dependent on the specific inhibitory mechanism of each allelochemical. These allelochemicals act on multiple target sites.
英文摘要	The allelopathic mechanism of submerged macrophytes was investigated on the cyanobacterium Microcystis aeruginosa and the green alga Pseudokirchneriella subcapitata. The effect of the allelochemicals pyrogallic acid (PA), gallic acid (GA), (+)-catechin (CA), caffeic acid (CFA), protocatechuic acid (PCA), nonanoic acid (NA) and cis-9-octadecenoic acid (COA) was evaluated by flow cytometry on esterase activity, membrane integrity, chlorophyll a fluorescence, cell volume and internal cell complexity. The highest sensitivity was observed for esterase activity in both test species. NA damaged the cell membrane and severely altered the internal cell complexity of both species. PA changed the esterase activity of both species and significantly decreased the fluorescence of chlorophyll a in M. aeruginosa cells (P < 0.05). Both GA and PCA inhibited the fluorescence of chlorophyll a and caused morphological changes in M. aeruginosa (P < 0.05). CFA inhibited the esterase activity, fluorescence of chlorophyll a and cell volume in M. aeruginosa at 50 and 100 mg L-1 concentrations; In P. subcapitata, the fluorescence of chlorophyll a and cell volume was inhibited at 50 mg L-1 but was stimulated at 100 mg L-1. COA changed the esterase activity. CA at 100 mg L-1 slightly inhibited the fluorescence of chlorophyll a in M. aeruginosa and stimulated the esterase activity in P. subcapitata. The responses observed for each parameter investigated were highly dependent on the specific inhibitory mechanism of each allelochemical. These allelochemicals act on multiple target sites.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Agronomy
研究领域[WOS]	Agriculture
关键词[WOS]	BLUE-GREEN-ALGAE ; MYRIOPHYLLUM-SPICATUM ; FLOW-CYTOMETRY ; GROWTH-INHIBITION ; MICROCYSTIS-AERUGINOSA ; CYANOBACTERIA ; MARINE ; COPPER ; FLUORESCENCE ; POLYPHENOLS
收录类别	SCI
资助信息	National Natural Science Foundation of China [30870221, 50808172]; Major Science and Technology Program for Water Pollution Control and Treatment of China [2012ZX07101007-005]; Foundation for Innovative Research Groups of the Natural Science Foundation of Hubei Province, China [2010CDA066]; National Science Foundation for Distinguished Young Scholars of Hubei Province, China [2010CDA093]
语种	英语
WOS记录号	WOS:000313824400015
公开日期	2013-10-31
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19331]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wang, J.,Zhu, J. Y.,Gao, Y. N.,et al. Toxicity of allelochemicals released by submerged macrophytes on phytoplankton[J]. ALLELOPATHY JOURNAL,2013,31(1):199-209.
APA	Wang, J..,Zhu, J. Y..,Gao, Y. N..,Liu, B. Y..,Liu, S. P..,...&Wu, Z. B..(2013).Toxicity of allelochemicals released by submerged macrophytes on phytoplankton.ALLELOPATHY JOURNAL,31(1),199-209.
MLA	Wang, J.,et al."Toxicity of allelochemicals released by submerged macrophytes on phytoplankton".ALLELOPATHY JOURNAL 31.1(2013):199-209.