Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout

doi:10.1016/j.bios.2021.113923

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout
	Gao, Xueqing 1,4; Jiang, Tianjia 4; Qin, Wei 2,3,4
刊名	BIOSENSORS & BIOELECTRONICS
	2022-03-15
卷号	200 页码:7
关键词	Potentiometry Aptasensor Electrogenerated chemiluminescence Escherichia coli
ISSN号	0956-5663
DOI	10.1016/j.bios.2021.113923
通讯作者	Jiang, Tianjia(tjjiang@yic.ac.cn) ; Qin, Wei(wqin@yic.ac.cn)
英文摘要	We introduce here a versatile approach to read out potentiometric aptasensors by electrogenerated chemiluminescence (ECL), which can amplify the small potential changes induced by the bacterial concentrations via ECL signals. In the present system, the electrode modified with single-walled carbon nanotubes (SWCNTs) and aptamer molecules acts as the reference electrode and is placed in the sample solution for sensing the bacterial concentration changes, while the Ru(bpy)(3)(2+) modified gold electrode serves as the working electrode for generating ECL signals and is placed in the detection solution containing tripropylamine (TPA) spatially separated from the sample solution by a salt bridge. Ru(bpy)(3)(2+) is immobilized on the gold electrode's surface for enhancement of luminous efficiency and reduction of reagent consumption. A moving-part-free fluid flowing system is introduced to promote the mass transport of TPA from the detection solution to the surface of the ECL generating electrode. When a constant potential is imposed between the working and reference electrodes, the potential changes at the SWCNTs-aptamer modified electrode induced by the bacterial concentrations can modulate the potentials at the Ru(bpy)(3)(2+) modified electrode, thus generating the ECL signals. The developed sensing strategy shows a highly sensitive response to E. coli O157: H7 in the linear range of 5-1000 CFU mL(-1) with a low detection limit of 2 CFU mL(-1). We believe that the proposed approach is promising to develop aptasensors for sensitive detection of bacterial cells.
WOS关键词	WALLED CARBON NANOTUBES ; IMMEDIATE DETECTION ; BACTERIA ; ELECTROCHEMILUMINESCENCE ; RELEASE ; SYSTEM
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种	英语
WOS记录号	WOS:000772286100009
资助机构	National Natural Science Foundation of China ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences ; Taishan Scholar Program of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31357]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Jiang, Tianjia; Qin, Wei
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China 4.Chinese Acad Sci, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, YICCAS,Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Gao, Xueqing,Jiang, Tianjia,Qin, Wei. Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout[J]. BIOSENSORS & BIOELECTRONICS,2022,200:7.
APA	Gao, Xueqing,Jiang, Tianjia,&Qin, Wei.(2022).Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout.BIOSENSORS & BIOELECTRONICS,200,7.
MLA	Gao, Xueqing,et al."Potentiometric aptasensing of Escherichia coli based on electrogenerated chemiluminescence as a highly sensitive readout".BIOSENSORS & BIOELECTRONICS 200(2022):7.