Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea

doi:10.1016/j.fgb.2015.01.007

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	Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea
	An, Bang 1; Li, Boqiang ; Qin, Guozheng ; Tian, Shiping
刊名	FUNGAL GENETICS AND BIOLOGY
	2015
卷号	75 页码:46-55
关键词	Rho3 Botrytis cinerea Virulence ROS Mitochondria
ISSN号	1087-1845
DOI	10.1016/j.fgb.2015.01.007
文献子类	Article
英文摘要	Small GTPases of the Rho family play an important role in regulating biological processes in fungi. In this study, we mainly investigated the biological functions of Rho3 in Botrytis cinerea, and found that deletion of the rho3 from B. cinerea significantly suppressed vegetative growth and conidiation, reduced appressorium formation and decreased virulence. Microscopy analysis revealed that the distance between septa was increased in the Delta rho3 mutant. In addition, mitochondria were suggested to be the main sources of intracellular reactive oxygen species (ROS) in B. cinerea based on dual staining with 2',7'-dichlorodihydro-fluorescein diacetate and MitoTracker orange. The Arho3 mutant showed less accumulation of ROS in the hyphae tips compared to the WT strain of B. cinerea. These results provide the novel evidence to ascertain the function of small GTPase Rho3 in regulating growth, conidiation and virulence of B. cinerea. (C) 2015 Elsevier Inc. All rights reserved.
学科主题	Genetics & Heredity ; Mycology
电子版国际标准刊号	1096-0937
出版地	SAN DIEGO
WOS关键词	GENERATING NADPH OXIDASES ; POLARIZED CELL-GROWTH ; ASPERGILLUS-NIDULANS ; MITOCHONDRIA ; PATHOGENICITY ; METABOLISM ; PROTEINS ; DISEASE ; GERMINATION ; ACTIVATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000350781000005
资助机构	973 program [2013CB127103] ; National Natural Science Foundation of China [31030051, 31172005]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25814]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Being 100049, Peoples R China
推荐引用方式 GB/T 7714	An, Bang,Li, Boqiang,Qin, Guozheng,et al. Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea[J]. FUNGAL GENETICS AND BIOLOGY,2015,75:46-55.
APA	An, Bang,Li, Boqiang,Qin, Guozheng,&Tian, Shiping.(2015).Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea.FUNGAL GENETICS AND BIOLOGY,75,46-55.
MLA	An, Bang,et al."Function of small GTPase Rho3 in regulating growth, conidiation and virulence of Botrytis cinerea".FUNGAL GENETICS AND BIOLOGY 75(2015):46-55.