Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum

doi:10.1016/j.ijfoodmicro.2015.05.007

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	Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum
	Zong, Yuanyuan 2; Li, Boqiang ; Tian, Shiping
刊名	INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
	2015
卷号	206 页码:102-108
关键词	Penicillium expansum Patulin biosynthesis Carbon source Nitrogen source Ambient pH
ISSN号	0168-1605
DOI	10.1016/j.ijfoodmicro.2015.05.007
文献子类	Article
英文摘要	Patulin, a potent mycotoxin which can cause serious health concerns, is mainly produced in foods by Penicillium expansum. Environmental factors play important roles in regulating biosynthesis of mycotoxins; however, information about the effects of environmental factors on patulin production and the involved mechanisms in P. expaasum is limited. Here, we investigated the effects of different carbon (C) and nitrogen (N) sources, and ambient pH on patulin production in three P. expansum strains T01, M1 and Pe21, and the expression profile of 15 genes involved in patulin biosynthetic pathway. It was found that C and N sources and pH had great influence on patulin production in P. expansum. In general, patulin production of all three P. expansum strains showed similar trends under different C and N sources and pH conditions, though there were some differences in the optimal conditions among these strains. Glucose-containing sugars, complex N sources, and acidic conditions were favorable conditions for patulin production. The results of RT-qPCR showed that the relative expressions of most of the patulin genes were up-regulated under patulin-permissive conditions, indicating that patulin biosynthesis was mainly regulated at transcriptional level by these environmental factors. These findings will provide useful information to better understand the regulation mechanisms of patulin biosynthesis, and be helpful in developing effective means for controlling patulin contamination. (C) 2015 Elsevier B.V. All rights reserved.
学科主题	Food Science & Technology ; Microbiology
电子版国际标准刊号	1879-3460
出版地	AMSTERDAM
WOS关键词	SECONDARY METABOLISM ; ASPERGILLUS-PARASITICUS ; BIOSYNTHESIS ; AFLATOXIN ; STERIGMATOCYSTIN ; MECHANISMS ; REPRESSION ; GROWTH ; APPLE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000358100500015
资助机构	National High Technology Research (863) Program of China [2012AA101607] ; National Natural Science Foundation of China [31171765, 31030051]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25573]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Zong, Yuanyuan,Li, Boqiang,Tian, Shiping. Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum[J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,2015,206:102-108.
APA	Zong, Yuanyuan,Li, Boqiang,&Tian, Shiping.(2015).Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum.INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY,206,102-108.
MLA	Zong, Yuanyuan,et al."Effects of carbon, nitrogen and ambient pH on patulin production and related gene expression in Penicillium expansum".INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 206(2015):102-108.