Biochemical and structural study of Arabidopsis hexokinase 1

doi:10.1107/S1399004714026091

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Biochemical and structural study of Arabidopsis hexokinase 1
	Feng, Juan 2; Zhao, Shun 2; Chen, Xuemin 2; Wang, Wenda ; Dong, Wei 2; Chen, Jinghua 2; Shen, Jian-Ren ; Liu, Lin ; Kuang, Tingyun
刊名	ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
	2015
卷号	71 页码:367-375
ISSN号	1399-0047
DOI	10.1107/S1399004714026091
文献子类	Article
英文摘要	Hexokinase 1 from Arabidopsis thaliana (AtHXK1) plays a dual role in glycolysis and sugar sensing for vital metabolic and physiological processes. The uncoupling of glucose signalling from glucose metabolism was demonstrated by the analysis of two mutants (AtHXK1(G104D) and AtHXK1(S177A)) that are catalytically inactive but still functional in signalling. In this study, substrate-binding experiments indicate that the two catalytically inactive mutants have a high affinity for glucose, and an ordered substrate-binding mechanism has been observed for wild-type AtHXK1. The structure of AtHXK1 was determined both in its inactive unliganded form and in its active glucose-bound form at resolutions of 1.8 and 2.0 angstrom, respectively. These structures reveal a domain rearrangement of AtHXK1 upon glucose binding. The 2.1 angstrom resolution structure of AtHXK1(S177A) in the glucose-bound form shows similar glucose-binding interactions as the wild type. A glucose-sensing network has been proposed based on these structures. Taken together, the results provide a structural explanation for the dual functions of AtHXK1.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
出版地	HOBOKEN
WOS关键词	SKELETAL-MUSCLE HEXOKINASE ; X-RAY CRYSTALLOGRAPHY ; YEAST-HEXOKINASE ; CRYSTAL-STRUCTURE ; ALLOSTERIC REGULATION ; ISOTOPE-EXCHANGE ; MOLECULAR-BASIS ; GLUCOSE ; GLUCOKINASE ; COMPLEX
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY-BLACKWELL
WOS记录号	WOS:000349439900020
资助机构	National Basic Research Program of China [2011CBA00901] ; National Natural Science Foundation of China [31170688] ; Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25785]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Feng, Juan,Zhao, Shun,Chen, Xuemin,et al. Biochemical and structural study of Arabidopsis hexokinase 1[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2015,71:367-375.
APA	Feng, Juan.,Zhao, Shun.,Chen, Xuemin.,Wang, Wenda.,Dong, Wei.,...&Kuang, Tingyun.(2015).Biochemical and structural study of Arabidopsis hexokinase 1.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,71,367-375.
MLA	Feng, Juan,et al."Biochemical and structural study of Arabidopsis hexokinase 1".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 71(2015):367-375.