Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis

doi:10.1007/s11120-015-0131-z

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	Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis
	Yang, Zhipan ; Shang, Zengzhen 2; Wang, Lei 2; Lu, Qingtao ; Wen, Xiaogang ; Chi, Wei ; Zhang, Lixin ; Lu, Congming
刊名	PHOTOSYNTHESIS RESEARCH
	2015
卷号	126 期号:2-3 页码:285-300
关键词	Arabidopsis ATase2 Chloroplast development Chloroplast gene expression Purine biosynthesis
ISSN号	0166-8595
DOI	10.1007/s11120-015-0131-z
文献子类	Article
英文摘要	To investigate the molecular mechanism of chloroplast biogenesis and development, we characterized an Arabidopsis mutant (dg169, delayed greening 169) which showed growth retardation and delayed greening phenotype in leaves. Newly emerged chlorotic leaves recovered gradually with leaf development in the mutant, and the mature leaves showed similar phenotype to those of wild-typewild-type plants. Compared with wild-type, the chloroplasts were oval-shaped and smaller and the thylakoid membranes were less abundant in yellow section of young leaves of dg169. In addition, the functions of photosystem II (PSII) and photosystem I (PSI) were also impaired. Furthermore, the amount of core subunits of PSII and PSI, as well as PSII and PSI complexes reduced in yellow section of young leaves of dg169. Map-based positional cloning identified that phenotype of dg169 was attributed to a point mutation of ATase2 which converts the conserved Ile-155 residue to Asn. ATase2 catalyzes the first step of de novo purine biosynthesis. This mutation resulted in impaired purine synthesis and a significant decrease in ATP, ADP, GTP and GDP contents. The analysis of ATase2-GFP protein fusion showed that ATase2 was localized to nucleoid of chloroplasts. Our results further demonstrated that the levels of PEP-dependent transcripts in yellow section of young leaves of dg169 were decreased while NEP-dependent and both PEP- and NEP-dependent transcripts and chloroplast DNA replications were increased. The results in this study suggest that ATase2 plays an essential role in early chloroplast development through maintaining PEP function.
学科主题	Plant Sciences
电子版国际标准刊号	1573-5079
出版地	DORDRECHT
WOS关键词	ACTIVE CHROMOSOME COMPLEX ; RNA-POLYMERASE ; PLASTID CHROMOSOME ; LEAF VARIEGATION ; PHOTOSYSTEM-II ; MESSENGER-RNA ; POTATO-TUBERS ; SIGMA FACTORS ; THALIANA ; PROTEIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000362006900009
资助机构	State Key Basic Research and Development Plan of China [2015CB150105] ; Chinese Academy of Sciences [KGZD-EW-T05]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25743]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yang, Zhipan,Shang, Zengzhen,Wang, Lei,et al. Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis[J]. PHOTOSYNTHESIS RESEARCH,2015,126(2-3):285-300.
APA	Yang, Zhipan.,Shang, Zengzhen.,Wang, Lei.,Lu, Qingtao.,Wen, Xiaogang.,...&Lu, Congming.(2015).Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis.PHOTOSYNTHESIS RESEARCH,126(2-3),285-300.
MLA	Yang, Zhipan,et al."Purine biosynthetic enzyme ATase2 is involved in the regulation of early chloroplast development and chloroplast gene expression in Arabidopsis".PHOTOSYNTHESIS RESEARCH 126.2-3(2015):285-300.