Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice)

doi:10.1093/jxb/eru405

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	Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice)
	Lu, Qingtao ; Ding, Shunhua ; Reiland, Sonja 2; Roediger, Anja 4; Roschitzki, Bernd 5; Xue, Peng 3; Gruissem, Wilhelm 2,5; Lu, Congming ; Baginsky, Sacha 2,4
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2015
卷号	66 期号:1 页码:175-187
关键词	Casein kinase II chloroplast mass spectrometry phosphorylation substrates transcriptionally active chromosome
ISSN号	0022-0957
DOI	10.1093/jxb/eru405
文献子类	Article
英文摘要	Plastid casein kinase II is an important regulator of transcription, posttranscriptional processes, and, most likely, different metabolic functions in dicotyledonous species. Here we report the identification and characterization of pCKII from the monocotyledonous species Oryza sativa. OspCKII activity was enriched from isolated rice chloroplasts using heparin-Sepharose chromatography, in which it co-elutes with the transcriptionally active chromosome (TAC) and several ribosomal proteins. Inclusion mass scanning of the kinase-active fraction identified the gene model for OspCKII. Transient expression of GFP fused to the 184 N-terminal amino acids of the OspCKII sequence in rice confirmed the chloroplastic localization of the kinase. OspCKII activity shows the characteristic features of casein kinase II, such as the utilization of GTP as phosphate donor, inhibition by low concentrations of heparin and poly-lysine, and utilization of the canonical pCKII motif E-S-E-G-E in the model substrate RNP29. Phosphoproteome analysis of a protein extract from rice leaves combined with a meta-analysis with published phosphoproteomics data revealed differences in the target protein spectrum between rice and Arabidopsis. Consistently, several pCKII phosphorylation sites in dicotyledonous plants are not conserved in monocots and algae, suggesting that details of pCKII regulation in plastids have changed during evolution.
学科主题	Plant Sciences
电子版国际标准刊号	1460-2431
出版地	OXFORD
WOS关键词	MUSTARD SINAPIS-ALBA ; PLASTID TRANSCRIPTION KINASE ; GENE-EXPRESSION ; PROTEIN-KINASE ; PHOSPHORYLATION NETWORKS ; RNA-POLYMERASE ; ARABIDOPSIS ; CK2 ; PROTEOMICS ; COMPLEXES
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000351660900015
资助机构	Chinese State Scholarship Program [[2006]3036] ; National Natural Science Foundation of China NSFC [31071336] ; Institute of Botany, Chinese Academy of Sciences ; Swiss National Science Foundation [SNF 31003A-127202] ; Deutsche Forschungsgemeinschaft (DFG) [Ba1902/2-1] ; ETH Zurich
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25661]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Biophys, Lab Prot & Peptide Pharmaceut, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China 3.Univ Zurich, ETH Zurich, Funct Genom Ctr Zurich, CH-8057 Zurich, Switzerland 4.ETH, Dept Biol, Plant Biotechnol, CH-8092 Zurich, Switzerland 5.Univ Halle Wittenberg, Inst Biochem & Biotechnol, D-06120 Halle, Saale, Germany
推荐引用方式 GB/T 7714	Lu, Qingtao,Ding, Shunhua,Reiland, Sonja,et al. Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice)[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(1):175-187.
APA	Lu, Qingtao.,Ding, Shunhua.,Reiland, Sonja.,Roediger, Anja.,Roschitzki, Bernd.,...&Baginsky, Sacha.(2015).Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice).JOURNAL OF EXPERIMENTAL BOTANY,66(1),175-187.
MLA	Lu, Qingtao,et al."Identification and characterization of chloroplast casein kinase II from Oryza sativa (rice)".JOURNAL OF EXPERIMENTAL BOTANY 66.1(2015):175-187.