JACALIN-LECTIN LIKE1 Regulates the Nuclear Accumulation of GLYCINE-RICH RNA-BINDING PROTEIN7, Influencing the RNA Processing of FLOWERING LOCUS C Antisense Transcripts and Flowering Time in Arabidopsis

doi:10.1104/pp.15.00801

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	JACALIN-LECTIN LIKE1 Regulates the Nuclear Accumulation of GLYCINE-RICH RNA-BINDING PROTEIN7, Influencing the RNA Processing of FLOWERING LOCUS C Antisense Transcripts and Flowering Time in Arabidopsis
	Xiao, Jun 2; Li, Chunhua 2; Xu, Shujuan 2; Xing, Lijing ; Xu, Yunyuan ; Chong, Kang 3
刊名	PLANT PHYSIOLOGY
	2015
卷号	169 期号:3 页码:2102-2117
ISSN号	0032-0889
DOI	10.1104/pp.15.00801
文献子类	Article
英文摘要	Lectins selectively recognize sugars or glycans for defense in living cells, but less is known about their roles in the development process and the functional network with other factors. Here, we show that Arabidopsis (Arabidopsis thaliana) JACALIN-LECTIN LIKE1 (AtJAC1) functions in flowering time control. Loss of function of AtJAC1 leads to precocious flowering, whereas overexpression of AtJAC1 causes delayed flowering. AtJAC1 influences flowering through regulation of the key flowering repressor gene FLOWERING LOCUS C (FLC). Genetic analysis revealed that AtJAC1's function is mostly dependent on GLYCINE-RICH RNA-BINDING PROTEIN7 (GRP7), an upstream regulator of FLC. Biochemical and cell biological data indicated that AtJAC1 interacted physically with GRP7 specifically in the cytoplasm. AtJAC1 influences the nucleocytoplasmic distribution of GRP7, with predominant nuclear localization of GRP7 when AtJAC1 function is lost but retention of GRP7 in the cytoplasm when AtJAC1 is overexpressed. A temporal inducible assay suggested that AtJAC1's regulation of flowering could be compromised by the nuclear accumulation of GRP7. In addition, GRP7 binds to the antisense precursor messenger RNA of FLC through a conserved RNA motif. Loss of GRP7 function leads to the elevation of total FLC antisense transcripts and reduced proximal-distal polyadenylation ratio, as well as histone methylation changes in the FLC gene body region and increased total functional sense FLC transcript. Attenuating the direct binding of GRP7 with competing artificial RNAs leads to changes of FLC antisense precursor messenger RNA processing and flowering transition. Taken together, our study indicates that AtJAC1 coordinates with GRP7 in shaping plant development through the regulation of RNA processing in Arabidopsis.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	PRE-MESSENGER-RNA ; PLANT-LECTINS ; ESCHERICHIA-COLI ; STRESS RESPONSES ; DEFENSE PROTEINS ; ABIOTIC STRESS ; THALIANA ; GENE ; FLC ; VERNALIZATION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000367393900049
资助机构	major state basic research program of China (973 Program) [2011CB915404] ; China-Netherlands Joint Scientific Thematic Research Program [GJHZ1309]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25697]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Natl Ctr Plant Gene Res, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Jun,Li, Chunhua,Xu, Shujuan,et al. JACALIN-LECTIN LIKE1 Regulates the Nuclear Accumulation of GLYCINE-RICH RNA-BINDING PROTEIN7, Influencing the RNA Processing of FLOWERING LOCUS C Antisense Transcripts and Flowering Time in Arabidopsis[J]. PLANT PHYSIOLOGY,2015,169(3):2102-2117.
APA	Xiao, Jun,Li, Chunhua,Xu, Shujuan,Xing, Lijing,Xu, Yunyuan,&Chong, Kang.(2015).JACALIN-LECTIN LIKE1 Regulates the Nuclear Accumulation of GLYCINE-RICH RNA-BINDING PROTEIN7, Influencing the RNA Processing of FLOWERING LOCUS C Antisense Transcripts and Flowering Time in Arabidopsis.PLANT PHYSIOLOGY,169(3),2102-2117.
MLA	Xiao, Jun,et al."JACALIN-LECTIN LIKE1 Regulates the Nuclear Accumulation of GLYCINE-RICH RNA-BINDING PROTEIN7, Influencing the RNA Processing of FLOWERING LOCUS C Antisense Transcripts and Flowering Time in Arabidopsis".PLANT PHYSIOLOGY 169.3(2015):2102-2117.