Subcellular Redistribution of Root Aquaporins Induced by Hydrogen Peroxide

doi:10.1016/j.molp.2015.02.017

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	Subcellular Redistribution of Root Aquaporins Induced by Hydrogen Peroxide
	Wudick, Michael M.; Li, Xiaojuan 4; Valentini, Valeria ; Geldner, Niko 3; Chory, Joanne 1; Lin, Jinxing 4; Maurel, Christophe ; Doan-Trung Luu
刊名	MOLECULAR PLANT
	2015
卷号	8 期号:7 页码:1103-1114
关键词	Arabidopsis aquaporin stress reactive oxygen species root
ISSN号	1674-2052
DOI	10.1016/j.molp.2015.02.017
文献子类	Article
英文摘要	Aquaporins are water channel proteins that mediate the fine-tuning of cell membrane water permeability during development or in response to environmental stresses. The present work focuses on the oxidative stress-induced redistribution of plasma membrane intrinsic protein (PIP) aquaporins from the plasma membrane (PM) to intracellular membranes. This process was investigated in the Arabidopsis root. Sucrose density gradient centrifugation showed that exposure of roots to 0.5 mM H2O2 induces significant depletion in PM fractions of several abundant PIP homologs after 15 min. Analyses by single-particle tracking and fluorescence correlative spectroscopy showed that, in the PM of epidermal cells, H2O2 treatment induces an increase in lateral motion and a reduction in the density of a fluorescently tagged form of the prototypal AtPIP2; 1 isoform, respectively. Co-expression analyses of AtPIP2;1 with endomembrane markers revealed that H2O2 triggers AtPIP2;1 accumulation in the late endosomal compartments. Lifetime analyses established that the high stability of PIPs was maintained under oxidative stress conditions, suggesting that H2O2 triggers a mechanism for intracellular sequestration of PM aquaporins without further degradation. In addition to information on cellular regulation of aquaporins, this study provides novel and complementary insights into the dynamic remodeling of plant internal membranes during oxidative stress responses.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号	1752-9867
出版地	CAMBRIDGE
WOS关键词	PLASMA-MEMBRANE AQUAPORINS ; WATER TRANSPORT ; VESICLE TRAFFICKING ; ARABIDOPSIS ROOTS ; PLANT-CELLS ; ENDOCYTOSIS ; PROTEIN ; REVEALS ; STRESS ; EFFLUX
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	CELL PRESS
WOS记录号	WOS:000357615600011
资助机构	Marie Curie Research Training Network Va-TEP [MRTN-CT-2006-035833]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25580]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland 2.Salk Inst Biol Studies, Plant Mol & Cellular Biol Lab, La Jolla, CA 92037 USA 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 4.Univ Montpellier 2, Montpellier SupAgro, UMR INRA 0386,Inst Biol Integrat Plantes, Biochim & Physiol Mole Plantes,UMR CNRS 5004, F-34060 Montpellier 2, France
推荐引用方式 GB/T 7714	Wudick, Michael M.,Li, Xiaojuan,Valentini, Valeria,et al. Subcellular Redistribution of Root Aquaporins Induced by Hydrogen Peroxide[J]. MOLECULAR PLANT,2015,8(7):1103-1114.
APA	Wudick, Michael M..,Li, Xiaojuan.,Valentini, Valeria.,Geldner, Niko.,Chory, Joanne.,...&Doan-Trung Luu.(2015).Subcellular Redistribution of Root Aquaporins Induced by Hydrogen Peroxide.MOLECULAR PLANT,8(7),1103-1114.
MLA	Wudick, Michael M.,et al."Subcellular Redistribution of Root Aquaporins Induced by Hydrogen Peroxide".MOLECULAR PLANT 8.7(2015):1103-1114.