Non-canonical structure, function and phylogeny of the B-sister MADS-box gene OsMADS30 of rice (Oryza sativa)

doi:10.1111/tpj.13055

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	Non-canonical structure, function and phylogeny of the B-sister MADS-box gene OsMADS30 of rice (Oryza sativa)
	Schilling, Susanne ; Gramzow, Lydia ; Lobbes, Dajana ; Kirbis, Alexander ; Weilandt, Lisa ; Hoffmeier, Andrea ; Junker, Astrid ; Weigelt-Fischer, Kathleen ; Klukas, Christian 1; Wu, Feng 2
刊名	PLANT JOURNAL
	2015
卷号	84 期号:6 页码:1059-1072
关键词	Oryza neo-functionalization neutral evolution helitron domestication imaging-based plant phenotyping
ISSN号	0960-7412
DOI	10.1111/tpj.13055
文献子类	Article
英文摘要	B-sister MADS-box genes play key roles in female reproductive organ and seed development throughout seed plants. This view is supported by their high conservation in terms of sequence, expression and function. In grasses, there are three subclades of B-sister genes: the OsMADS29-, the OsMADS30-and the OsMADS31-like genes. Here, we report on the evolution of the OsMADS30-like genes. Our analyses indicate that these genes evolved under relaxed purifying selection and are rather weakly expressed. OsMADS30, the representative of the OsMADS30-like genes from rice (Oryza sativa), shows strong sequence deviations in its 30 region when compared to orthologues from other grass species. We show that this is due to a 2.4-kbp insertion, possibly of a hitherto unknown helitron, which confers a heterologous C-terminal domain to OsMADS30. This putative helitron is not present in the OsMADS30 orthologues from closely related wild rice species, pointing to a relatively recent insertion event. Unlike other B-sister mutants O. sativa plants carrying a T-DNA insertion in the OsMADS30 gene do not show aberrant seed phenotypes, indicating that OsMADS30 likely does not have a canonical 'B-sister function'. However, imaging-based phenotyping of the T-DNA carrying plants revealed alterations in shoot size and architecture. We hypothesize that sequence deviations that accumulated during a period of relaxed selection in the gene lineage that led to OsMADS30 and the alteration of the C-terminal domain might have been a precondition for a potential neo-functionalization of OsMADS30 in O. sativa.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	MULTIPLE SEQUENCE ALIGNMENT ; FLORAL HOMEOTIC GENES ; SEGMENTAL DUPLICATIONS ; GENOME SEQUENCE ; EVOLUTION ; TRANSCRIPTION ; PROTEINS ; REVEALS ; MAP ; DNA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000368268700002
资助机构	Deutsche Forschungsgemeinschaft [TH 417/7-1] ; National Bioresource Project, MEXT, Japan
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25578]
专题	中科院植物分子生理学重点实验室
作者单位	1.Univ Jena, Dept Genet, D-07743 Jena, Germany 2.Leibniz Inst Plant Genet & Crop Plant Res IPK, Mol Genet, D-06466 Gatersleben, Germany 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Schilling, Susanne,Gramzow, Lydia,Lobbes, Dajana,et al. Non-canonical structure, function and phylogeny of the B-sister MADS-box gene OsMADS30 of rice (Oryza sativa)[J]. PLANT JOURNAL,2015,84(6):1059-1072.
APA	Schilling, Susanne.,Gramzow, Lydia.,Lobbes, Dajana.,Kirbis, Alexander.,Weilandt, Lisa.,...&Theissen, Guenter.(2015).Non-canonical structure, function and phylogeny of the B-sister MADS-box gene OsMADS30 of rice (Oryza sativa).PLANT JOURNAL,84(6),1059-1072.
MLA	Schilling, Susanne,et al."Non-canonical structure, function and phylogeny of the B-sister MADS-box gene OsMADS30 of rice (Oryza sativa)".PLANT JOURNAL 84.6(2015):1059-1072.