Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification

doi:10.1016/j.bse.2015.07.013

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	Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification
	Lu, Youyuan 3; Cheng, Tao 2; Zhu, Tiantian ; Jiang, Dan 3; Zhou, Shiliang 2; Jin, Ling ; Yuan, Qingjun 3; Huang, Luqi 3
刊名	BIOCHEMICAL SYSTEMATICS AND ECOLOGY
	2015
卷号	61 页码:488-492
关键词	Angelica sinensis Medicinal plant Microsatellite Molecular marker Cross-amplification Population genetics
ISSN号	0305-1978
DOI	10.1016/j.bse.2015.07.013
文献子类	Article
英文摘要	Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the Hardy-Weinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis. (C) 2015 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
电子版国际标准刊号	1873-2925
出版地	OXFORD
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
WOS记录号	WOS:000361164800068
资助机构	National Natural Science Foundation of China (NSFC) [81360615, 81274027, 81130070] ; Beijing Joint Project Specific Funds ; Independent Project of Basic Scientific Research Operating Expenses of Institute of Chinese Materia Medial, China Academy of Chinese Medical Sciences [2011ZDXK-01]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25821]
专题	系统与进化植物学国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 2.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Daodi Herbs, Beijing 100700, Peoples R China 3.Gansu Univ Chinese Med, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Lu, Youyuan,Cheng, Tao,Zhu, Tiantian,et al. Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification[J]. BIOCHEMICAL SYSTEMATICS AND ECOLOGY,2015,61:488-492.
APA	Lu, Youyuan.,Cheng, Tao.,Zhu, Tiantian.,Jiang, Dan.,Zhou, Shiliang.,...&Huang, Luqi.(2015).Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification.BIOCHEMICAL SYSTEMATICS AND ECOLOGY,61,488-492.
MLA	Lu, Youyuan,et al."Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification".BIOCHEMICAL SYSTEMATICS AND ECOLOGY 61(2015):488-492.