Callicarpa japonica Thunb. attenuates cigarette smoke-induced neutrophil inflammation and mucus secretion

doi:10.1016/j.jep.2015.08.056

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	Callicarpa japonica Thunb. attenuates cigarette smoke-induced neutrophil inflammation and mucus secretion
	Lee, Jae-Won ; Shin, Na-Rae ; Park, Ji-Won ; Park, So-Yeon ; Kwon, Ok-Kyoung ; Lee, Han-Sol ; Kim, Jung Hee ; Lee, Hee Jae ; Lee, Joongku ; Zhang, Zhi-yun
刊名	JOURNAL OF ETHNOPHARMACOLOGY
	2015
卷号	175 页码:1-8
关键词	Callicarpa japonica Thunb. Cigarette smoke Inflammation Neutrophil MUC5AC
ISSN号	0378-8741
DOI	10.1016/j.jep.2015.08.056
文献子类	Article
英文摘要	Ethnopharmacological relevance: Callicarpa japonica Thunb. (CJT) is traditionally used as an herbal remedy for the treatment of inflammatory diseases in Korea, China, and Japan. In this study, we evaluated the effects of C. japonica Thunb. (CJT) on the development of COPD using a Cigarette smoke (CS)-induced murine model and cigarette smoke condensate (CSC)-stimulated H292 cells, human pulmonary mucoepidermoid cell line. Material and methods: C japonica Thunb. was isolated from the leaves and stem of C japonica. The methanol extract profile was obtained by UPLC Q-TOF-MS analysis. In in vivo experiment, the mice received 1 h of cigarette smoke for 10 days. C japonica Thunb. was administered to mice by oral gavage 1 h before cigarette smoke exposure for 10 days. In in vitro experiment, we evaluated the effect of C. japonica Thunb. on the expression of MUC5AC and proinflammatory cytokines in H292 cells stimulated with CSC. Results: CJT treatment effectively suppressed the infiltration of neutrophils, and decreased the production of ROS and the activity of neutrophil elastase in the bronchoalveolar lavage fluid (BALF) induced by CS. CJT also significantly attenuated production of proinflammatory cytokines such as IL-6 and TNF-alpha in the BALF, and reduced the infiltration of inflammatory cells and the production of mucus in lung tissue induced by CS. In in vitro experiments, CJT decreased the expression of MUC5AC and proinflammatory cytokines in CSC-stimulated H292 cells. Furthermore, CJT attenuated the phosphorylation of ERIC induced by CSC in H292 cells. Taken together, CJT effectively reduced the neutrophil airway inflammation and mucus secretion induced by CS in murine model, and inhibited the expression of MUC5AC in CSC-stimulated H292 human lung cell line. These findings suggest that CJT has a therapeutic potential for the treatment of COPD. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
学科主题	Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
电子版国际标准刊号	1872-7573
出版地	CLARE
WOS关键词	OBSTRUCTIVE PULMONARY-DISEASE ; AIRWAY INFLAMMATION ; MUC5AC EXPRESSION ; MODEL ; KINASE ; CELLS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER IRELAND LTD
WOS记录号	WOS:000366765600001
资助机构	Ministry of Science, ICT and Future Planning [FGC 1011433] ; KRIBB Research Initiative Program of the Republic of Korea [KGM 1221521]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25670]
专题	系统与进化植物学国家重点实验室
作者单位	1.Kangwon Natl Univ, Coll Med, Dept Pharmacol, Chunchon 200701, Kangwon, South Korea 2.[Lee, Jae-Won 3.Shin, Na-Rae 4.Park, Ji-Won 5.Park, So-Yeon 6.Kwon, Ok-Kyoung 7.Lee, Han-Sol 8.Kim, Jung Hee 9.Oh, Sei-Ryang 10.Korea Res Inst Biosci & Biotechnol, Nat Med Res Ctr, Chungbuk 363883, South Korea
推荐引用方式 GB/T 7714	Lee, Jae-Won,Shin, Na-Rae,Park, Ji-Won,et al. Callicarpa japonica Thunb. attenuates cigarette smoke-induced neutrophil inflammation and mucus secretion[J]. JOURNAL OF ETHNOPHARMACOLOGY,2015,175:1-8.
APA	Lee, Jae-Won.,Shin, Na-Rae.,Park, Ji-Won.,Park, So-Yeon.,Kwon, Ok-Kyoung.,...&Ahn, Kyung-Seop.(2015).Callicarpa japonica Thunb. attenuates cigarette smoke-induced neutrophil inflammation and mucus secretion.JOURNAL OF ETHNOPHARMACOLOGY,175,1-8.
MLA	Lee, Jae-Won,et al."Callicarpa japonica Thunb. attenuates cigarette smoke-induced neutrophil inflammation and mucus secretion".JOURNAL OF ETHNOPHARMACOLOGY 175(2015):1-8.