Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging | |
Zhang, Song4; Jing, Jiangbo4; Meng, Lingchen4; Xu, Bin4; Ma, Xibo1,2,3; Tian, Wenjing4 | |
刊名 | CHEMOSENSORS |
2022-05-01 | |
卷号 | 10期号:5页码:10 |
关键词 | hepatocellular carcinoma (HCC) Glypican-3 aggregation-induced emission (AIE) cells imaging |
DOI | 10.3390/chemosensors10050195 |
通讯作者 | Ma, Xibo(xibo.ma@ia.ac.cn) ; Tian, Wenjing(wjtian@jlu.edu.cn) |
英文摘要 | Hepatocellular carcinoma (HCC) is a malignant tumor with high morbidity and mortality on a global scale, and the development of accurate detection and imaging methods for HCC cells is urgently needed. Herein, by connecting peptide L5, which can specifically bind to the overexpressed Glypican-3 (GPC-3) protein of HCC cells with aggregation-induced emission (AIE) moiety ammonium cation-functionalized 9,10-distyrylanthracene (NDSA) via the "click" reaction, we synthesized a fluorescent probe NDSA-L5. In an aqueous solution, the probe shows weak emission, whereas, in the presence of the GPC-3 protein, bright fluorescence can be obtained since NDSA-L5 binds to the GPC-3 protein, leading to the restricted intramolecular movement of AIE-active NDSA-L5. The imaging and flow cytometry experiments demonstrate that the NDSA-L5 probe can rapidly accumulate in the subcutaneous HCC cells and liver tumor tissue and shows a potential application in early detection and surgical navigation for HCC cancer. |
资助项目 | Natural Science Foundation of China[21835001] ; Natural Science Foundation of China[52073116] ; Natural Science Foundation of China[51773080] ; Natural Science Foundation of China[82090051] ; Natural Science Foundation of China[81871442] ; JLU Science and Technology Innovative Research Team[2021TD-03] ; National Key Research programs of China[2016YFA0100900] ; National Key Research programs of China[2016YFA0100902] ; Youth Innovation Promotion Association CAS[Y201930] |
WOS关键词 | PHOTODYNAMIC THERAPY ; LIVER-CANCER ; LABEL-FREE |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
语种 | 英语 |
出版者 | MDPI |
WOS记录号 | WOS:000801364700001 |
资助机构 | Natural Science Foundation of China ; JLU Science and Technology Innovative Research Team ; National Key Research programs of China ; Youth Innovation Promotion Association CAS |
内容类型 | 期刊论文 |
源URL | [http://ir.ia.ac.cn/handle/173211/49539] |
专题 | 自动化研究所_模式识别国家重点实验室_生物识别与安全技术研究中心 |
通讯作者 | Ma, Xibo; Tian, Wenjing |
作者单位 | 1.Univ Chinese Acad Sci, Sch Artificial Intelligence, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Automat, NLPR, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Automat, CBSR, Beijing 100049, Peoples R China 4.Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Song,Jing, Jiangbo,Meng, Lingchen,et al. Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging[J]. CHEMOSENSORS,2022,10(5):10. |
APA | Zhang, Song,Jing, Jiangbo,Meng, Lingchen,Xu, Bin,Ma, Xibo,&Tian, Wenjing.(2022).Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging.CHEMOSENSORS,10(5),10. |
MLA | Zhang, Song,et al."Peptide-Conjugated Aggregation-Induced Emission Fluorogenic Probe for Glypican-3 Protein Detection and Hepatocellular Carcinoma Cells Imaging".CHEMOSENSORS 10.5(2022):10. |
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