<p>Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus<br></p>

doi:10.1016/j.fsi.2022.03.043

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源利用所重点实验室

	Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus
	Wang, Mengmei 4; Lv, Chengjie 2,3; Chen, Yuying 2,3; Bi, Xiujuan 2,3; Yang, Dinglong 2,3; Zhao, Jianmin 1,2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2022-05-01
卷号	124 页码:12-20
关键词	Apostichopus japonicus Bacillus subtilis Growth performance Digestive enzyme Immunity Intestinal flora
ISSN号	1050-4648
DOI	10.1016/j.fsi.2022.03.043
通讯作者	Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要	In the present study, a potential probiotic Bacillus subtilis D1-2 with antibacterial activity was isolated from the gut of Apostichopus japonicus. The purpose of this experiment was to assess the effect of B. subtilis D1-2 at different concentrations (C: 0 CFU/g, BL: 10(5) CFU/g, BM: 10(7) CFU/g and BH: 10(9) CFU/g) on the growth performance, digestive enzyme activity, immune ability and intestinal flora of A. japonicus. After the 56-day feeding trial, the final body weight and weight gain rate of juvenile sea cucumber A. japonicus fed B. subtilis D1-2 were significantly increased, especially in the BM group. Additionally, the lipase activity of the intestine was significantly increased in the BM and BH groups. Enhanced immunity was also found in sea cucumbers supplemented with B. subtilis D1-2. Alpha diversity indices showed that the B. subtilis D1-2-supplemented groups had higher intestinal microbial richness and diversity than the control group. The beta diversity analysis indicated that the bacterial communities in the B. subtilis D1-2-supplemented groups were quite similar but different from the bacterial communities in the control group. Dietary supplementation with B. subtilis D1-2 increased the relative abundance of some potential probiotic-related genera, including Lactobacillus, Clostridium, Lactococcus, Bifidobacterium and Streptococcus. In conclusion, dietary addition of B. subtilis D1-2 could effectively promote the growth of A. japonicus, improve its digestion and immunity capacity to a certain extent, and actively regulate the intestinal microflora of A. japonicus.
WOS关键词	MICROBIOTA ; SUPPLEMENTATION ; PARAMETERS ; RESISTANCE ; EXPRESSION ; GENES
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
WOS记录号	WOS:000793042900002
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31059]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
通讯作者	Yang, Dinglong
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China
推荐引用方式 GB/T 7714	Wang, Mengmei,Lv, Chengjie,Chen, Yuying,et al. Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus [J]. FISH & SHELLFISH IMMUNOLOGY,2022,124:12-20.
APA	Wang, Mengmei,Lv, Chengjie,Chen, Yuying,Bi, Xiujuan,Yang, Dinglong,&Zhao, Jianmin.(2022). Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus .FISH & SHELLFISH IMMUNOLOGY,124,12-20.
MLA	Wang, Mengmei,et al." Effects of the potential probiotic Bacillus subtilis D1-2 on growth, digestion, immunity and intestinal flora in juvenile sea cucumber, Apostichopus japonicus ".FISH & SHELLFISH IMMUNOLOGY 124(2022):12-20.