A self-powered rotating paper-based analytical device for sensing of thrombin

doi:10.1016/j.snb.2021.130917

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A self-powered rotating paper-based analytical device for sensing of thrombin
	Li, Qingling 5; Xu, Yuehan 5; Qi, Ji 1,4; Zheng, Xiaoli 5; Liu, Shudi 3; Lin, Dong 2,4; Zhang, Liangwei 4; Liu, Ping 4,5; Li, Bowei 4; Chen, Lingxin 4
刊名	SENSORS AND ACTUATORS B-CHEMICAL
	2022-01-15
卷号	351 页码:8
关键词	Microfluidic paper-based analytical devices MuPAD Biofuel cell Electrochemical Self-powered
DOI	10.1016/j.snb.2021.130917
通讯作者	Li, Bowei(bwli@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	We firstly constructed a kind of self-powered rotating paper-based microfluidic chip and used it to detect thrombin. The integrated movable valve has the ability to control the fluid user-friendly on microfluidic paper-based analytical devices (mu PADs), and this advantage let it convenient to implement the complex multistep operations. In addition, this technique is compatible and versatile to the self-powered mu PADs at differing levels of complexity. To obtain the supercapacitor signal, the DNA sequence (DNA1) and a glucose oxidase (GOx) modified DNA sequence (GOx-DNA2) were linked with aptamer to form the sandwich structure. Then thrombin will combine with its specific aptamer by strong binding ability, and the GOx-DNA2 could be released and to catalyze the oxidation of glucose. Because of GOx-triggered reaction, the voltage can be generated to charge the paper supercapacitor and its signal could be monitored by digital multimeter. The assay shows good performance with wide linear range (3-1350 nM) and the limit of detection for the thrombin assay was down to 0.9 nM. The proposed rotating paper-based aptamer device can integrate the complex self-powered multistep operations, and show good potential to be applied for on-site assay in the field of diagnosis.
WOS关键词	BIOFUEL CELL ; LOW-COST ; MICROFLUIDICS ; BIOSENSOR ; PLATFORM ; RELEASE ; GLUCOSE ; DRIVEN ; BLOOD
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种	英语
WOS记录号	WOS:000817967900003
资助机构	National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation Key Project, China ; Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31017]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Li, Bowei; Chen, Lingxin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China 3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264005, Peoples R China 4.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 5.Shandong Normal Univ, Collaborat Innovat Ctr Functionalized Probes Chem, Coll Chem Chem Engn & Mat Sci,Univ Shandong, Inst Mol & Nano Sci,Key Lab Mol & Nano Probes,Min, Jinan 250014, Peoples R China
推荐引用方式 GB/T 7714	Li, Qingling,Xu, Yuehan,Qi, Ji,et al. A self-powered rotating paper-based analytical device for sensing of thrombin[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,351:8.
APA	Li, Qingling.,Xu, Yuehan.,Qi, Ji.,Zheng, Xiaoli.,Liu, Shudi.,...&Chen, Lingxin.(2022).A self-powered rotating paper-based analytical device for sensing of thrombin.SENSORS AND ACTUATORS B-CHEMICAL,351,8.
MLA	Li, Qingling,et al."A self-powered rotating paper-based analytical device for sensing of thrombin".SENSORS AND ACTUATORS B-CHEMICAL 351(2022):8.