Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway

doi:10.1021/acsomega.1c06892

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	Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway
	Xu, Duoling 4,5; Wang, Chao 4,5; Wu, Jie 4,5; Fu, Yuanxiang 2,3; Li, Shujun 1; Hou, Wentao 1; Lin, Ling 4,5; Li, Pei 4,5; Yu, Dongsheng 4,5; Zhao, Wei 4,5
刊名	ACS OMEGA
	2022-04-26
卷号	7 期号:16 页码:13546-13556
ISSN号	2470-1343
DOI	10.1021/acsomega.1c06892
通讯作者	Yu, Dongsheng(yudsh@mail.sysu.edu.cn) ; Zhao, Wei(zhaowei3@mail.sysu.edu.cn)
英文摘要	Graphene oxide quantum dots (GOQDs) are considered to be a new method for regulating the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). However, there are few reports on such regulation with different concentrations of GOQDs, and the molecular mechanism has not been fully elucidated. The purposes of this study were, first, to explore the effects of GOQDs on the proliferation and differentiation of BMSCs in vitro and in vivo, and, second, to provide a theoretical basis for the repair of bone defects. Live/Dead staining, EdU staining, immuno-fluorescence staining, alkaline phosphatase (ALP), western blotting, and qT-PCR were used for detecting the proliferation and differentiation of BMSCs after coculture with GOQDs of different concentrations. Hematoxylin and eosin (HE) staining and Van Gieson (VG) staining were used to detect new bone regeneration in vivo. The results showed that low-concentration GOQDs (0.1 and 1 mu g/mL) promoted the proliferation and differentiation of BMSCs. Compared with the 1 mu g/mL GOQD group, the 0.1 mu g/mL GOQD group had better ability to promote the proliferation and differentiation of BMSCs. HE and VG staining results showed the greatest proportion of new bone area on sandblasted, large-grit, and acid-etched (SLA)/GOQD scaffolds. Furthermore, the ratio of active beta-catenin and the phosphorylation level of GSK-3 beta (p-GSK-3 beta) increased after BMSCs treatment with 0.1 mu g/mL GOQDs. Low concentrations of GOQDs improved the osteogenic differentiation ability of BMSCs by activating the Wnt/beta-catenin signaling pathway.
资助项目	National Natural Science Foundation of China[81974146] ; National Natural Science Foundation of China[81873711] ; Science and Technology Planning Project of Guangdong Province[2016A020215094] ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation (Climbing Program Special Funds)[pdjh 2022b0012]
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000812740100001
资助机构	National Natural Science Foundation of China ; Science and Technology Planning Project of Guangdong Province ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation (Climbing Program Special Funds)
内容类型	期刊论文
源URL	[http://ir.imr.ac.cn/handle/321006/174392]
专题	金属研究所_中国科学院金属研究所
通讯作者	Yu, Dongsheng; Zhao, Wei
作者单位	1.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China 2.Guizhou Inst Technol, Guizhou Prov Key Lab Energy Chem, Guiyang 550003, Peoples R China 3.Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China 4.Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou 510050, Guangdong, Peoples R China 5.Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Xu, Duoling,Wang, Chao,Wu, Jie,et al. Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway[J]. ACS OMEGA,2022,7(16):13546-13556.
APA	Xu, Duoling.,Wang, Chao.,Wu, Jie.,Fu, Yuanxiang.,Li, Shujun.,...&Zhao, Wei.(2022).Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway.ACS OMEGA,7(16),13546-13556.
MLA	Xu, Duoling,et al."Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway".ACS OMEGA 7.16(2022):13546-13556.