The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida

doi:10.3390/pathogens11040464

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida
	Liu, Xin 1,2,3,4; Wang, Xinhui 1,2; Sun, Boguang 1,2,3; Sun, Li 1,2,3,4
刊名	PATHOGENS
	2022-04-01
卷号	11 期号:4 页码:16
关键词	Edwardsiella piscicida thiamine transporter c-di-GMP virulence
DOI	10.3390/pathogens11040464
通讯作者	Sun, Boguang(sunboguang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
英文摘要	Edwardsiella piscicida is a pathogenic bacterium, which can infect a number of fish species and cause a disease termed edwardsiellosis, threatening global fish farming with high prevalence and mortality. Thiamine (Vitamin B1), functioning in the form of thiamine pyrophosphate (TPP), is essential for almost all organisms. Bacteria acquire TPP by biosynthesis or by transportation of exogenous thiamine. TPP availability has been associated with bacterial pathogenicity, but the underlying mechanisms remain to be discovered. The role of thiamine in the pathogenicity of E. piscicida is unknown. In this study, we characterized a thiamine transporter (TT) operon in E. piscicida. The deletion of the TT operon resulted in an intracellular TPP lacking situation, which led to attenuated overall pathogenicity, impaired abilities associated with motility and host cell adhesion, as well as decreased expression of certain flagellar and adhesion genes. Moreover, TPP starvation led to intracellular c-di-GMP reduction, and introducing into the TPP-suppressed mutant strain an exogenous diguanylate cyclase for c-di-GMP synthesis restored the virulence loss. Taken together, this work reveals the involvement of thiamine uptake in the virulence regulation of E. piscicida, with c-di-GMP implicated in the process. These finding could be employed to explore potential drug targets against E. piscicida.
资助项目	National Key R&D Program of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Microbiology
语种	英语
出版者	MDPI
WOS记录号	WOS:000786725800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178882]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Boguang; Sun, Li
作者单位	1.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, CAS Ctr Ocean Megasci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266003, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Xin,Wang, Xinhui,Sun, Boguang,et al. The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida[J]. PATHOGENS,2022,11(4):16.
APA	Liu, Xin,Wang, Xinhui,Sun, Boguang,&Sun, Li.(2022).The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida.PATHOGENS,11(4),16.
MLA	Liu, Xin,et al."The Involvement of Thiamine Uptake in the Virulence of Edwardsiella piscicida".PATHOGENS 11.4(2022):16.