Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip

doi:10.1080/10584587.2022.2061203

	Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip
	Huang, Chenghong 3; Tang, Hong 3; Chen, Hongjie 3; Huang, Xiaorong 3; Fang, Ying 3; Wang, KangRui 3; Men, Rongrong 3; Gao, Jie 3; Wang, Yang 3; Chen, Yanyan 2
刊名	INTEGRATED FERROELECTRICS
	2022-06-13
卷号	226 期号:1 页码:148-155
关键词	protein chip alpha-fetoprotein site-directed immobilization of antibody
ISSN号	1058-4587
DOI	10.1080/10584587.2022.2061203
通讯作者	Huang, Chenghong(chhuang2007@sinano.ac.cn) ; Chen, Yanyan(yychen2006@sinano.ac.cn) ; Jin, Gang(gajin@imech.ac.cn)
英文摘要	For immunoassay, immobilization of antibody is the most important technique in bioengineering for maintaining its intrinsic activity of antigen binding. The sugar chain in the Fc region carried by antibody can be oxidized into aldehyde group by sodium m-periodate, which can couple with the amino-group on chemistry modified substrate surface through covalent linking. Thereby, in situ site-directed immobilization of the oxidized antibody for only one-step reaction can be performed. Here we propose a facile method that oxidized anti-human alpha-fetoprotein antibody (anti-AFP) was in situ site-directed immobilized on APTES treated silicon substrate utilizing microfluidic channel system for AFP immunoassay. The shape of the immobilized anti-AFP was observed by AFM and imaged by imaging ellipsometry of self-developed protein system. Results shown that the amount of antibody immobilization amount and capacity of antigen binding of random immobilization and site-directed immobilization were separately enhanced 16.0% and 7.0%. the suggested strategy has the advantages of simple, environment-friend and convenient-operation.
资助项目	NSF of Chongqing[cstc2019jscx-msxm0366] ; Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism[GYFJWSW-201905] ; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission[KJZD-K201806401] ; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission[KJZD-K201906401] ; graduate Innovation Fund of CQUST[YKJCX2020509] ; graduate Innovation Fund of CQUST[YKJCX2120502] ; graduate Innovation Fund of CQUST[YKJCX2120541] ; graduate Innovation Fund of CQUST[202111551006]
WOS关键词	ORIENTATION ; STRATEGY
WOS研究方向	Engineering ; Physics
语种	英语
WOS记录号	WOS:000805668600015
资助机构	NSF of Chongqing ; Open Fund of Chongqing Key Laboratory of Industrial Fermentation Microorganism ; Project of Scientific and Technological Research Program of Chongqing Municipal Education Commission ; graduate Innovation Fund of CQUST
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/89636]
专题	力学研究所_国家微重力实验室
通讯作者	Huang, Chenghong; Chen, Yanyan; Jin, Gang
作者单位	1.Chinese Acad Sci, Inst Mech, Beijing, Peoples R China 2.Chinese Acad Sci, Suzhou Inst Nanotech & Nanobion, Suzhou, Peoples R China 3.CQUST, Chongqing Key Lab Ind Fermentat Microorganism, Chongqing, Peoples R China
推荐引用方式 GB/T 7714	Huang, Chenghong,Tang, Hong,Chen, Hongjie,et al. Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip[J]. INTEGRATED FERROELECTRICS,2022,226(1):148-155.
APA	Huang, Chenghong.,Tang, Hong.,Chen, Hongjie.,Huang, Xiaorong.,Fang, Ying.,...&Jin, Gang.(2022).Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip.INTEGRATED FERROELECTRICS,226(1),148-155.
MLA	Huang, Chenghong,et al."Comparison of Random and Site-Directed Immobilization of Antibody for alpha-Fetoprotein Direct Immunoassay by Protein Chip".INTEGRATED FERROELECTRICS 226.1(2022):148-155.