An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake

doi:10.1111/nph.13637

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake
	He, Zhenyan ; Yan, Huili 1; Chen, Yanshan 1; Shen, Hongling 1; Xu, Wenxiu ; Zhang, Haiyan ; Shi, Lei ; Zhu, Yong-Guan ; Ma, Mi
刊名	NEW PHYTOLOGIST
	2016
卷号	209 期号:2 页码:746-761
关键词	aromatic/arginine (Ar/R) arsenic (As) arsenite (As(III)) aquaporin Pteris vittata PvTIP4 1 uptake
ISSN号	0028-646X
DOI	10.1111/nph.13637
文献子类	Article
英文摘要	The fern Pteris vittata is an arsenic hyperaccumulator. The genes involved in arsenite (As (III)) transport are not yet clear. Here, we describe the isolation and characterization of a new P. vittata aquaporin gene, PvTIP4;1, which may mediate As(III) uptake. PvTIP4;1 was identified from yeast functional complement cDNA library of P. vittata. Arsenic toxicity and accumulating activities of PvTIP4;1 were analyzed in Saccharomyces cerevisiae and Arabidopsis. Subcellular localization of PvTIP4;1-GFP fusion protein in P. vittata protoplast and callus was conducted. The tissue expression of PvTIP4; 1 was investigated by quantitative real-time PCR. Site-directed mutagenesis of the PvTIP4;1 aromatic/arginine (Ar/R) domain was studied. Heterologous expression in yeast demonstrates that PvTIP4;1 was able to facilitate As(III) diffusion. Transgenic Arabidopsis showed that PvTIP4;1 increases arsenic accumulation and induces arsenic sensitivity. Images and FM4-64 staining suggest that PvTIP4;1 localizes to the plasma membrane in P. vittata cells. A tissue location study shows that PvTIP4;1 transcripts are mainly expressed in roots. Site-directed mutation in yeast further proved that the cysteine at the LE1 position of PvTIP4;1 Ar/R domain is a functional site. PvTIP4;1 is a new represented tonoplast intrinsic protein (TIP) aquaporin from P. vittata and the function and location results imply that PvTIP4;1 may be involved in As(III) uptake.
学科主题	Plant Sciences
电子版国际标准刊号	1469-8137
出版地	HOBOKEN
WOS关键词	MAJOR INTRINSIC PROTEINS ; ARSENATE REDUCTASE ; VACUOLAR MEMBRANE ; ARABIDOPSIS ; GLYCEROL ; FERN ; SELECTIVITY ; TRANSPORT ; FAMILY ; CHANNEL
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000373376500029
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370299, 31470549]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25279]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	He, Zhenyan,Yan, Huili,Chen, Yanshan,et al. An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake[J]. NEW PHYTOLOGIST,2016,209(2):746-761.
APA	He, Zhenyan.,Yan, Huili.,Chen, Yanshan.,Shen, Hongling.,Xu, Wenxiu.,...&Ma, Mi.(2016).An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake.NEW PHYTOLOGIST,209(2),746-761.
MLA	He, Zhenyan,et al."An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake".NEW PHYTOLOGIST 209.2(2016):746-761.