Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis

doi:10.1007/s00299-015-1910-x

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	Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis
	Yuan, Yangyang 4; Fang, Linchuan 4; Karungo, Sospeter Karanja 4; Zhang, Langlang 4; Gao, Yingying 4; Li, Shaohua 1,3; Xin, Haiping 1,3
刊名	PLANT CELL REPORTS
	2016
卷号	35 期号:3 页码:655-666
关键词	GRAS transcription factor VaPAT1 Vitis amurensis Abiotic stress Gibberellic acid (GA) Stress-related genes
ISSN号	0721-7714
DOI	10.1007/s00299-015-1910-x
文献子类	Article
英文摘要	The plant-specific GRAS transcription factor family regulates diverse processes involved in plant growth, development and stress responses. In this study, VaPAT1, a GRAS gene from Vitis amurensis was isolated and functionally characterized. Sequence alignment and phylogenetic analysis showed that VaPAT1 has a high sequence identity to CmsGRAS and OsCIGR1, which belong to PAT1 branch of GRAS family and function in stress resistance. The transcription of VaPAT1 was markedly induced by stress-related phytohormone abscisic acid (ABA) and various abiotic stress treatments such as cold, drought and high salinity, however, it was repressed by exogenous gibberellic acid (GA) application. Overexpression of VaPAT1 increased the cold, drought and high salinity tolerance in transgenic Arabidopsis. When compared with wild type (WT) seedlings, the VaPAT1-overexpression lines accumulated higher levels of proline and soluble sugar under these stress treatments. Moreover, stress-related genes such as AtSIZ1, AtCBF1, AtATR1/MYB34, AtMYC2, AtCOR15A, AtRD29A and AtRD29B showed higher expression levels in VaPAT1 transgenic lines than in WT Arabidopsis under normal growth conditions. Together, our results indicated that VaPAT1 functions as a positive transcriptional regulator involved in grapevine abiotic stress responses.
学科主题	Plant Sciences
电子版国际标准刊号	1432-203X
出版地	NEW YORK
WOS关键词	SIGNAL-TRANSDUCTION ; ABSCISIC-ACID ; DROUGHT TOLERANCE ; COLD TOLERANCE ; GENE FAMILY ; GIBBERELLIN RESPONSES ; EXPRESSION ANALYSIS ; TRANSGENIC RICE ; SALT STRESSES ; THALIANA
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000371241100013
资助机构	National Science Foundation of China (NSFC)National Natural Science Foundation of China (NSFC) [31130047, 31471857] ; National Key Technology R&D Program of the Ministry of Science and Technology during the Twelfth Five-year Plan Period [2013BAD02B04-1] ; Youth Innovation Promotion Association of CAS [2015281]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25272]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Yangyang,Fang, Linchuan,Karungo, Sospeter Karanja,et al. Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis[J]. PLANT CELL REPORTS,2016,35(3):655-666.
APA	Yuan, Yangyang.,Fang, Linchuan.,Karungo, Sospeter Karanja.,Zhang, Langlang.,Gao, Yingying.,...&Xin, Haiping.(2016).Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis.PLANT CELL REPORTS,35(3),655-666.
MLA	Yuan, Yangyang,et al."Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis".PLANT CELL REPORTS 35.3(2016):655-666.