iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence

doi:10.1016/j.jprot.2016.06.031

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院北方资源植物重点实验室

	iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence
	Liu, Ruiling 1; Wang, Yuying ; Qin, Guozheng ; Tian, Shiping 1
刊名	JOURNAL OF PROTEOMICS
	2016
卷号	146 页码:80-89
关键词	Fruit senescence Vacuolar membrane iTRAQ-NanoLC-MS/MS Transporters Membrane trafficking
ISSN号	1874-3919
DOI	10.1016/j.jprot.2016.06.031
文献子类	Article
英文摘要	The vacuole is by far the largest multifunctional organelle in fruits and plays a functional role in fruit development and fruit quality. Despite its significance, little information exists pertaining to the role of the vacuolar membrane (tonoplast) in the process of fruit senescence. In the present study, an iTRAQ-based quantitative proteomic approach was used to characterize the dynamic alterations in the tonoplast proteome during fruit senescence. Tonoplasts were purified from apple fruit at various stages of senescence using an iodixanol step gradient protocol. A total of 345 tonoplast-related proteins were identified with diverse functions such as transporters and proton pumps, signal transduction, membrane fusion or vesicle trafficking, cellular metabolic process, defense response, protein folding and degradation, and cytoskeleton. Changes in protein abundance during storage were characterized for the identified proteins. A total of 22 proteins displayed differential levels of abundance during storage. The senescence-related tonoplast proteins mostly function in the transportation of metabolites, signal transduction, membrane trafficking, and stress response. RT-qPCR analysis was used to quantify the level of expression of nine genes encoding some of the differentially abundant proteins. The results of this study provide new information regarding the function of the tonoplast during fruit senescence. Biological significance: Studies on the postharvest physiology and biochemistry of apple fruit have been conducted for several decades. Little proteomic information is available, however, pertaining to the role of the vacuole in fruit ripening and senescence. In the present study, an iTRAQ-based quantitative proteomic analysis was conducted on tonoplasts isolated from apple fruit in order to gain a global view of alterations in the tonoplast proteome during fruit senescence. The information obtained in the present study not only provides basic information about the tonoplast proteome in apple fruit but also characterized the quantitative changes that occur in the abundance of tonoplast proteins during the course of fruit senescence. This study provides a deeper insight into the cellular functions of the vacuole during fruit senescence that can serve as a basis for the development of future biotechnological strategies for the improvement of fruit quality. (C) 2016 Elsevier B.V. All rights reserved.
学科主题	Biochemical Research Methods
电子版国际标准刊号	1876-7737
出版地	AMSTERDAM
WOS关键词	CONTROLLED-ATMOSPHERE ; ARABIDOPSIS-THALIANA ; PLANT VACUOLES ; TOMATO FRUIT ; PEACH FRUIT ; APPLE FRUIT ; PROTEINS ; STORAGE ; EXPRESSION ; TRANSPORTERS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000383942600008
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015B100604]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25237]
专题	中科院北方资源植物重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ruiling,Wang, Yuying,Qin, Guozheng,et al. iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence[J]. JOURNAL OF PROTEOMICS,2016,146:80-89.
APA	Liu, Ruiling,Wang, Yuying,Qin, Guozheng,&Tian, Shiping.(2016).iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence.JOURNAL OF PROTEOMICS,146,80-89.
MLA	Liu, Ruiling,et al."iTRAQ-based quantitative proteomic analysis reveals the role of the tonoplast in fruit senescence".JOURNAL OF PROTEOMICS 146(2016):80-89.