BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex

doi:10.1104/pp.16.00248

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	BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex
	Zhang, Lin 1; Duan, Zhikun 1; Zhang, Jiao ; Peng, Lianwei
刊名	PLANT PHYSIOLOGY
	2016
卷号	171 期号:2 页码:1291-1306
ISSN号	0032-0889
DOI	10.1104/pp.16.00248
文献子类	Article
英文摘要	Thylakoid membrane-localized chloroplast ATP synthases use the proton motive force generated by photosynthetic electron transport to produce ATP from ADP. Although it is well known that the chloroplast ATP synthase is composed of more than 20 proteins with alpha(3)beta(3)gamma(1)epsilon(1)delta 1I1II1III14IV1 stoichiometry, its biogenesis process is currently unclear. To unravel the molecular mechanisms underlying the biogenesis of chloroplast ATP synthase, we performed extensive screening for isolating ATP synthase mutants in Arabidopsis (Arabidopsis thaliana). In the recently identified bfa3 (biogenesis factors required for ATP synthase 3) mutant, the levels of chloroplast ATP synthase subunits were reduced to approximately 25% of wild-type levels. In vivo labeling analysis showed that assembly of the CF1 component of chloroplast ATP synthase was less efficient in bfa3 than in the wild type, indicating that BFA3 is required for CF1 assembly. BFA3 encodes a chloroplast stromal protein that is conserved in higher plants, green algae, and a few species of other eukaryotic algae, and specifically interacts with the CF1 beta subunit. The BFA3 binding site was mapped to a region in the catalytic site of CF1 beta. Several residues highly conserved in eukaryotic CF1 beta are crucial for the BFA3-CF1 beta interaction, suggesting a coevolutionary relationship between BFA3 and CF1 beta. BFA3 appears to function as a molecular chaperone that transiently associates with unassembled CF1 beta at its catalytic site and facilitates subsequent association with CF1 alpha during assembly of the CF1 subcomplex of chloroplast ATP synthase.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	ROCKVILLE
WOS关键词	CYCLIC ELECTRON FLOW ; COUPLING FACTOR-I ; ARABIDOPSIS-THALIANA ; PHOTOSYNTHETIC COMPLEX ; GAMMA-SUBUNIT ; BINDING-SITE ; PROTEIN ; F-1-ATPASE ; PROMOTES ; PHOTOPROTECTION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
WOS记录号	WOS:000380699200040
资助机构	National Natural Science FundsNational Natural Science Foundation of China (NSFC) [31322007] ; Hundred Talents Program of the Chinese Academy of SciencesChinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/25315]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, CAS Ctr Excellence Mol Plant Sci, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Lin,Duan, Zhikun,Zhang, Jiao,et al. BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex[J]. PLANT PHYSIOLOGY,2016,171(2):1291-1306.
APA	Zhang, Lin,Duan, Zhikun,Zhang, Jiao,&Peng, Lianwei.(2016).BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex.PLANT PHYSIOLOGY,171(2),1291-1306.
MLA	Zhang, Lin,et al."BIOGENESIS FACTOR REQUIRED FOR ATP SYNTHASE 3 Facilitates Assembly of the Chloroplast ATP Synthase Complex".PLANT PHYSIOLOGY 171.2(2016):1291-1306.