An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea

doi:10.1038/srep33364

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	An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea
	Liu, Yanling 2; Zhao, Zhongjuan 4; Xue, Zheyong 1; Wang, Long ; Cai, Yunfei ; Wang, Peng ; Wei, Tiandi ; Gong, Jing ; Liu, Zhenhua ; Li, Juan
刊名	SCIENTIFIC REPORTS
	2016
卷号	6
ISSN号	2045-2322
DOI	10.1038/srep33364
文献子类	Article
英文摘要	Paralogous members of the oxidosqualene cyclase (OSC) family encode a diversity of enzymes that are important in triterpenoid biosynthesis. This report describes the isolation of the Gentiana straminea gene GsAS2 that encodes a beta-amyrin synthase (beta AS) enzyme. Unlike its previously isolated paralog GsAS1, GsAS2 lacks introns. Its predicted protein product was is a 759 residue polypeptide that shares high homology with other known beta-amyrin synthases (beta ASs). Heterologously expressed GsAS2 generates more beta-amyrin in yeast than does GsAS1. Constitutive over-expression of GsAS2 resulted in a 5.7 fold increase in oleanolic acid accumulation, while over-expression of GsAS1 led to a 3 fold increase. Additionally, RNAi-directed suppression of GsAS2 and GsAS1 in G. straminea decreased oleonolic acid levels by 65.9% and 21% respectively, indicating that GsAS2 plays a more important role than GsAS1 in oleanolic acid biosynthesis in G. straminea. We uses a docking model to explore the catalytic mechanism of GsAS1/2 and predicted that GsAS2, with its Y560, have higher efficiency than GsAS1 and mutated versions of GsAS2 in beta-amyrin produce. When the key residue in GsAS2 was mutagenized, it produced about 41.29% and 71.15% less beta-amyrin than native, while the key residue in GsAS1 was mutagenized to that in GsAS2, the mutant produced 38.02% more beta-amyrin than native GsAS1.
学科主题	Multidisciplinary Sciences
出版地	LONDON
WOS关键词	EUPHORBIA-TIRUCALLI L ; MULTIFUNCTIONAL TRITERPENE SYNTHASE ; OXIDOSQUALENE CYCLASES ; ARABIDOPSIS-THALIANA ; FUNCTIONAL-ANALYSIS ; MAXIMUM-LIKELIHOOD ; SQUALENE SYNTHASE ; MOLECULAR-CLONING ; ESCHERICHIA-COLI ; PLANTS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000383575000001
资助机构	National Key Research and Development Program of China [2016YFD0101902] ; National High Technology Research and Development Program 863National High Technology Research and Development Program of China [2013AA102602-4] ; National Special Science Research Program of China [2013CB967303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31270328, 31471515, 31200226, 31500232, 31370337] ; National Transgenic Project of China [2013ZX08010002-002] ; China Postdoctoral Science FoundationChina Postdoctoral Science Foundation [2014M551893] ; Science & Technology Plan of Shandong Province [2013GNC11010] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2015PC009] ; Research Program for International S&T Cooperation Projects of Shandong Province [2011176]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/24695]
专题	中科院植物分子生理学重点实验室
作者单位	1.Shandong Acad Sci, Inst Ecol, Key Lab Appl Microbiol Shandong Prov, Jinan 250014, Peoples R China 2.Shandong Univ, Sch Life Sci, Minist Educ, Key Lab Plant Cell Engn & Germplasm Innovat, Jinan 250100, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Nanxincun 20,Fragrant Hill, Beijing 100093, Peoples R China 4.Laiwu Vocat & Tech Coll, Dept Informat Engn, Laiwu 271100, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yanling,Zhao, Zhongjuan,Xue, Zheyong,et al. An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea[J]. SCIENTIFIC REPORTS,2016,6.
APA	Liu, Yanling.,Zhao, Zhongjuan.,Xue, Zheyong.,Wang, Long.,Cai, Yunfei.,...&Xiang, Fengning.(2016).An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea.SCIENTIFIC REPORTS,6.
MLA	Liu, Yanling,et al."An Intronless beta-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea".SCIENTIFIC REPORTS 6(2016).