This study proposes an ultrafast laser ablation method for improving the depth uniformity of microgrooves in bursting discs. Under a lower laser fluence, the influence of the spot overlap rate on the depth uniformity of microgrooves was studied. The results show that 80% of the spot overlap ratio has good performance in ablation efficiency and depth uniformity of microgrooves. On this basis, the relationship between the number of laser scanning layers and the depth of microgrooves was studied, and the number of scanning layers needed to ablate 70 mu m microgrooves was obtained. Based on the combination of the process parameters and the optimization of the laser scanning path, laser ablation of bursting disc microgrooves with a specific shape was realized. The depth uniformity of microgrooves in different sections of the bursting disc was not worse than 4 mu m. The preliminary bursting test shows that the bursting pressure between the discs was no more than 0.06 Mpa. Compared with the results of the traditional processing method, the microgroove depth uniformity of bursting discs was greatly improved. Therefore, femtosecond laser ablation technology provides an advanced manufacturing method for bursting disc microgroove machining.