STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe

doi:10.1021/acs.analchem.1c03474

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	STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe
	G. Liu; G. Peng; J. Dai; R. Zhou; C. Wang; X. Yan; X. Jia; X. Liu; Y. Gao; L. Wang and G. Lu
刊名	Analytical Chemistry
	2021
卷号	93 期号:44 页码:14784-14791
ISSN号	32700
DOI	10.1021/acs.analchem.1c03474
英文摘要	Lipid droplets (LDs) are spherical organelles that participate in numerous biological processes. In order to visualize LDs on the nanoscale, nanoscopy fluorescence imaging is considered as the most attractive technique but is substantially limited by the characteristics of fluorescent probes. Thus, the development of a superior fluorescent probe that is capable of nanoscopy fluorescence imaging has attracted enormous attention. Herein, a benzodithiophene-tetraoxide-based molecule Lipi-BDTO has been developed that can easily undergo the stimulated emission depletion (STED) process and displays high photostability. These two characteristics of fluorescent probes finely satisfy the requirements of STED nanoscopy imaging. Indeed, applying the probe for STED imaging achieves a high resolution of 65 nm, belonging to one of the leading results of LDs fluorescence imaging. Furthermore, the high photostability of this fluorescent probe enables it to monitor the dynamics of LDs by time-lapse STED imaging as well as to visualize the three-dimensional (3D) spatial distribution of LDs by 3D STED imaging. Notably, the resolution of the 3D STED image represents one of the best LDs fluorescence imaging results so far. Besides STED nanoscopy imaging, the superior utility of this fluorescent probe has been also demonstrated in two-color 3D confocal imaging and four-color confocal imaging. 2021 American Chemical Society
URL标识	查看原文
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/65652]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	G. Liu,G. Peng,J. Dai,et al. STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe[J]. Analytical Chemistry,2021,93(44):14784-14791.
APA	G. Liu.,G. Peng.,J. Dai.,R. Zhou.,C. Wang.,...&L. Wang and G. Lu.(2021).STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe.Analytical Chemistry,93(44),14784-14791.
MLA	G. Liu,et al."STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe".Analytical Chemistry 93.44(2021):14784-14791.