A rapid parallel laser microdissection method

doi:10.19650/j.cnki.cjsi.J2107713

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	A rapid parallel laser microdissection method
	C. Huang; J. Xie; D. Yi; Y. Kang and F. Zhuang
刊名	Yi Qi Yi Biao Xue Bao/Chinese Journal of Scientific Instrument
	2021
卷号	42 期号:8
ISSN号	2543087
DOI	10.19650/j.cnki.cjsi.J2107713
英文摘要	With regard to the traditional laser microdissection using the relative motion between the single laser focus and the workpiece, there are limitations in forming the machining trajectory, such as the low cutting efficiency and the difficulty in closing the cutting trajectory. To address these issues, a laser microdissection method based on digital micromirror device without mechanical movement is proposed. By adjusting the digital micromirror array, the amplitude of the laser beam can be adjusted, and the arbitrary light field intensity distribution can be obtained in the sample area with the optical path design. In this way, the parallel cutting of plane projection is realized with almost the same intensity. Meanwhile, the process of key parameter design, simulation, and system construction is described. Specifically, the polyester film is first cut with various patterns such as straight line, hollow ring and hollow rectangle. Then, the frozen section of cell tissue is cut. Experiments show that, by one projection, designable patterned structures can be cut in parallel with accurate target graphics and high cutting efficiency. Under the 20 objective lens, the minimum cutting line width reaches sub-micron, and the minimum ring diameter is smaller than the single cell diameter. This method can provide new ideas and new directions for improving the efficiency and accuracy of the laser microdissection system. 2021, Science Press. All right reserved.
URL标识	查看原文
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/65571]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	C. Huang,J. Xie,D. Yi,et al. A rapid parallel laser microdissection method[J]. Yi Qi Yi Biao Xue Bao/Chinese Journal of Scientific Instrument,2021,42(8).
APA	C. Huang,J. Xie,D. Yi,&Y. Kang and F. Zhuang.(2021).A rapid parallel laser microdissection method.Yi Qi Yi Biao Xue Bao/Chinese Journal of Scientific Instrument,42(8).
MLA	C. Huang,et al."A rapid parallel laser microdissection method".Yi Qi Yi Biao Xue Bao/Chinese Journal of Scientific Instrument 42.8(2021).