Laser induced forward transfer isolating complex-shaped cell by beam shaping | |
P. Liang; L. Shang; Y. Wang; M. J. Booth and B. Li | |
刊名 | Biomedical Optics Express |
2021 | |
卷号 | 12期号:11页码:7024-7032 |
ISSN号 | 21567085 |
DOI | 10.1364/BOE.439054 |
英文摘要 | Beam shaping techniques have been widely used in holographic optical tweezers to accurately manipulate tiny particles and hologram optimization algorithms have also been widely reported to improve the optical trapping performance. In this paper, we presented a beam shaping laser induced forward transfer (BS-LIFT) technique to isolate complex-shaped cells. To do this, we built up a BS-LIFT instrument which combined beam shaping methods and laser induced forward transfer using liquid-crystal-on-silicon spatial light modulator. The laser beam was modulated into multiple desired points at the focal plane employing the GerchbergSaxton (GS) algorithm. Feasibility was verified through transferring various samples. To our knowledge, this is the first demonstration of BS-LIFT applied to the transfer complex-shaped cells. We successfully transferred cells whose size ranged from 1 m to 100 m. Our design will provide a novel approach for the application of this beam shaping technique and the isolation of single cells with variable shapes. 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
URL标识 | 查看原文 |
内容类型 | 期刊论文 |
源URL | [http://ir.ciomp.ac.cn/handle/181722/65373] |
专题 | 中国科学院长春光学精密机械与物理研究所 |
推荐引用方式 GB/T 7714 | P. Liang,L. Shang,Y. Wang,et al. Laser induced forward transfer isolating complex-shaped cell by beam shaping[J]. Biomedical Optics Express,2021,12(11):7024-7032. |
APA | P. Liang,L. Shang,Y. Wang,&M. J. Booth and B. Li.(2021).Laser induced forward transfer isolating complex-shaped cell by beam shaping.Biomedical Optics Express,12(11),7024-7032. |
MLA | P. Liang,et al."Laser induced forward transfer isolating complex-shaped cell by beam shaping".Biomedical Optics Express 12.11(2021):7024-7032. |
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