Controlling the Shrinkage of 3D Hot Spot Droplets as a Microreactor for Quantitative SERS Detection of Anticancer Drugs in Serum Using a Handheld Raman Spectrometer

doi:10.1021/acs.analchem.2c00071

	Controlling the Shrinkage of 3D Hot Spot Droplets as a Microreactor for Quantitative SERS Detection of Anticancer Drugs in Serum Using a Handheld Raman Spectrometer
	Zhou, Guoliang 1,2; Li, Pan 1,3; Ge, Meihong 1,2; Wang, Junping 3; Chen, Siyu 1,2; Nie, Yuman 4; Wang, Yaoxiong 4; Qin, Miao 1,2; Huang, Guangyao 1,2; Lin, Dongyue 1
刊名	ANALYTICAL CHEMISTRY
	2022-03-22
卷号	94
ISSN号	0003-2700
DOI	10.1021/acs.analchem.2c00071
通讯作者	Li, Pan(lipan2011@iim.ac.cn) ; Wang, Hongzhi(wanghz@hfcas.ac.cn) ; Yang, Liangbao(lbyang@iim.ac.cn)
英文摘要	Quantitative measurement is one of the ultimate targets for surface-enhanced Raman spectroscopy (SERS), but it suffers from difficulties in controlling the uniformity of hot spots and placing the target molecules in the hot spot space. Here, a convenient approach of three-phase equilibrium controlling the shrinkage of three-dimensional (3D) hot spot droplets has been demonstrated for the quantitative detection of the anticancer drug 5-fluorouracil (5-FU) in serum using a handheld Raman spectrometer. Droplet shrinkage, triggered by the shaking of aqueous nanoparticle (NP) colloids with immiscible oil chloroform (CHCI3) after the addition of negative ions and acetone, not only brings the nanoparticles in close proximity but can also act as a microreactor to enhance the spatial enrichment capability of the analyte in plasmonic sites and thereby realize simultaneously controlling 3D hot spots and placing target molecules in hot spots. Moreover, the shrinking process of Ag colloid droplets has been investigated using a high-speed camera, an in situ transmission electron microscope (in situ TEM), and a dark-field microscope (DFM), demonstrating the high stability and uniformity of nanoparticles in droplets. The shrunk Ag NP droplets exhibit excellent SERS sensitivity and reproducibility for the quantitative analysis of 5-FU over a large range of 50-1000 ppb. Hence, it is promising for quantitative analysis of complex systems and long-term monitoring of bioreactions.
资助项目	Research Instrument and Equipment Development Project, Chinese Academy of Sciences[YJKYYQ20200022] ; Anhui Provincial Key RD Program[202104d07020002] ; National Science Foundation of China[21974142]
WOS关键词	SPECTROSCOPY ; 5-FLUOROURACIL ; ARRAYS
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000779948100034
资助机构	Research Instrument and Equipment Development Project, Chinese Academy of Sciences ; Anhui Provincial Key RD Program ; National Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/128673]
专题	中国科学院合肥物质科学研究院
通讯作者	Li, Pan; Wang, Hongzhi; Yang, Liangbao
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Hlth & Med Technol, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Peoples R China 4.Chinese Acad Sci, Inst Intelligent Machines, Hefei Inst Phys Sci, Hefei 230031, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Guoliang,Li, Pan,Ge, Meihong,et al. Controlling the Shrinkage of 3D Hot Spot Droplets as a Microreactor for Quantitative SERS Detection of Anticancer Drugs in Serum Using a Handheld Raman Spectrometer[J]. ANALYTICAL CHEMISTRY,2022,94.
APA	Zhou, Guoliang.,Li, Pan.,Ge, Meihong.,Wang, Junping.,Chen, Siyu.,...&Yang, Liangbao.(2022).Controlling the Shrinkage of 3D Hot Spot Droplets as a Microreactor for Quantitative SERS Detection of Anticancer Drugs in Serum Using a Handheld Raman Spectrometer.ANALYTICAL CHEMISTRY,94.
MLA	Zhou, Guoliang,et al."Controlling the Shrinkage of 3D Hot Spot Droplets as a Microreactor for Quantitative SERS Detection of Anticancer Drugs in Serum Using a Handheld Raman Spectrometer".ANALYTICAL CHEMISTRY 94(2022).