Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans

doi:10.1039/d2bm00274d

	Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans
	Elsherbiny, Shereen M.1,2,3; Shao, Changsheng 1,2; Acheampong, Adolf 1,2; Khalifa, Mahmoud A.3,4; Liu, Chao 1,2; Huang, Qing 1,2
刊名	BIOMATERIALS SCIENCE
	2022-04-05
ISSN号	2047-4830
DOI	10.1039/d2bm00274d
通讯作者	Huang, Qing(huangq@ipp.ac.cn)
英文摘要	The clinical application of photodynamic therapy (PDT) is still limited because of the drawbacks of the traditional photosensitizers, such as low singlet oxygen (O-1(2)) quantum yield and the problem of photobleaching. Herein, carbon quantum dots (CQDs) derived from broccoli natural biomass as a carbon source were fabricated via a simple hydrothermal method and showed outstanding PDT ability as an effective photodynamic agent tested in Caenorhabditis elegans (C. elegans) models. The as-prepared broccoli-derived CQDs (BCQDs) showed excellent water solubility and optical properties and could generate singlet oxygen (O-1(2)) effectively under irradiated light with a wavelength of 660 nm. The in vivo experiment revealed that the PDT efficiency of the BCQDs was dependent on the induction of germline apoptosis through the cep-1/p53 pathway. Further investigation confirmed the DNA damage of the worm by the BCQDs after sufficient light irradiation, which was tested by measuring the egl-1-fold induction in hus-1(op244), and cep-1(w40) mutants that have a loss of function in the genes involved in DNA damage response such as hus-1 (DNA checkpoint gene) and cep-1/p53 (tumor suppressor). The lack of germline apoptosis in the loss of function mutants egl-1(n487), hus-1(op244), and cep-1(w40) exposed to light irradiation compared with the control proved the necessity of these genes in DNA damage-induced germline apoptosis. Therefore, this work has not only provided a new photodynamic agent but also introduced C. elegans as an easy and high-throughput model for the rapid evaluation of the efficiency of PDT.
资助项目	National Natural Science Foundation of China[11635013] ; CAS-TWAS President's Fellowship
WOS研究方向	Materials Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000785865300001
资助机构	National Natural Science Foundation of China ; CAS-TWAS President's Fellowship
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/128469]
专题	中国科学院合肥物质科学研究院
通讯作者	Huang, Qing
作者单位	1.Chinese Acad Sci, Inst Intelligent Machine, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei Inst Phys Sci,Anhui Key Lab Environm Toxico, Hefei 230031, Peoples R China 2.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei 230026, Peoples R China 3.Mansoura Univ, Fac Sci, Dept Phys, Mansoura 35516, Egypt 4.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Elsherbiny, Shereen M.,Shao, Changsheng,Acheampong, Adolf,et al. Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans[J]. BIOMATERIALS SCIENCE,2022.
APA	Elsherbiny, Shereen M.,Shao, Changsheng,Acheampong, Adolf,Khalifa, Mahmoud A.,Liu, Chao,&Huang, Qing.(2022).Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans.BIOMATERIALS SCIENCE.
MLA	Elsherbiny, Shereen M.,et al."Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans".BIOMATERIALS SCIENCE (2022).