Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy

doi:10.1039/c9nr07017f

CORC > 长春光学精密机械与物理研究所 > 中国科学院长春光学精密机械与物理研究所

	Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy
	X.W.Xu; M.L.Sun; D.D.Wang; W.H.Bu; Z.L.Wang; Y.Q.Shen
刊名	Nanoscale
	2019
卷号	11 期号:45 页码:21953-21963
关键词	gene-therapy,osteoblast differentiation,induce autophagy,delivery,expression,stimulation,osteoclasts,inhibition,vectors,cells,Chemistry,Science & Technology - Other Topics,Materials Science,Physics
ISSN号	2040-3364
DOI	10.1039/c9nr07017f
英文摘要	Gene therapy is one of the most common and effective ways for the regeneration of defective bone tissue, but even highly efficient gene delivery vectors are insufficient. In this study, bone morphogenetic protein-2 plasmid (pBMP-2) was encapsulated by polyethylenimine-modified porous silica nanoparticles (PPSNs), which were synthesized via an ethyl ether emulsion method. Owing to the high specific surface area and high absorption characteristics, low cytotoxicy PPSNs can efficiently load and protect pBMP-2. The resulting PPSN/pBMP-2 can transfect MC3T3-E1 cells effectively to promote osteogenic differentiation and increase calcium deposition in vitro. Interestingly, the mass of calcium deposition nodules decreased dur to the presence of an autophagy inhibitor, demonstrating that PPSNs stimulated the autophagy pathway. Because of their excellent biocompatibility, high transfection efficiency, and ability to stimulate autophagy, the as-prepared PPSN/pBMP-2 could efficiently transfect local cells in a defect area in vivo. Micro-computed tomography and histological images demonstrated that PPSN/pBMP-2 could efficiently promote new bone formation in a 5 mm sized rat calvarial defect model. Taken together, our newly synthesized PPSNs could efficiently carry pBMP-2 and deliver it to the target cells as well as stimulating the autophagy pathway, resulting in significant osteogenic differentiation and bone regeneration.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/62909]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	X.W.Xu,M.L.Sun,D.D.Wang,et al. Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy[J]. Nanoscale,2019,11(45):21953-21963.
APA	X.W.Xu,M.L.Sun,D.D.Wang,W.H.Bu,Z.L.Wang,&Y.Q.Shen.(2019).Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy.Nanoscale,11(45),21953-21963.
MLA	X.W.Xu,et al."Bone formation promoted by bone morphogenetic protein-2 plasmid-loaded porous silica nanoparticles with the involvement of autophagy".Nanoscale 11.45(2019):21953-21963.