Optogenetically engineered cell-based graphene transistor for pharmacodynamic evaluation of anticancer drugs | |
Yang J(杨佳)5,6,7; Li GX(李恭新)4; Zu LP(祖立鹏)5,6,7; Wang WX(王文学)6,7; Ge ZX(葛治星)5,6,7; Yang WG(杨文广)1; Zhong Y(仲亚)5,6,7; Zhang, Tianbiao2; Zhao, Ying3; Liu LQ(刘连庆)6,7 | |
刊名 | Sensors and Actuators B: Chemical |
2022 | |
卷号 | 358页码:1-11 |
关键词 | Cell-based biosensor Optogenetics Photosensitive proteins Cancer cells Graphene transistor Pharmacodynamic evaluation |
ISSN号 | 0925-4005 |
产权排序 | 1 |
英文摘要 | Rapid and effective cell-based pharmacodynamic evaluation in vitro is crucial for providing an experimental basis for the effectiveness and safety of drugs, and the existing cell-based methods for pharmacodynamics evaluation are usually invasive, dependent on chemical reagents, and/or unable to monitor the process in real time. Here, an optogenetically engineered cell-based graphene transistor is presented as a biosensor for the pharmacodynamic evaluation of anticancer drugs. The biosensor consists of a bare graphene transistor and optogenetically engineered cells as the gate terminal. The photoresponse of engineered cells regulates the output of the transistor and the increment pattern in the transistor output current upon drug action can be used to evaluate the drug efficacy. The results show that the optogenetic engineering of cancer cells does not affect the killing effect of drugs on the cells, and validate the effectiveness of the biosensor. The patterns of photoinduced increments exhibit significant variation with drug action time within 4 h or drug concentration in a range of 1 nM to 1 mM, and can qualitatively characterize the drug efficacy. Furthermore, the drug efficacy can be quantitatively evaluated with an indicator by logarithmically fitting the photoinduced increment patterns. The result also shows that the drain–source voltage significantly affects the evaluation performance and it is necessary to calibrate the voltage value to enhance the performance of the biosensor. The proposed biosensor affords simple, non-destructive, and time-efficient pharmacodynamic evaluation in vitro and is significant to understand the effect and mechanism of drugs in its early development stage. |
资助项目 | National Key R&D Program of China, China[2018YFB1304700] ; National Natural Sci-ence Foundation of China, China[U1908215] ; National Natural Sci-ence Foundation of China, China[61903157] ; National Natural Sci-ence Foundation of China, China[91848201] ; National Natural Sci-ence Foundation of China, China[61973316] ; Liaoning Revitalization Talents Pro-gram, China[XLYC2002014] |
WOS关键词 | FIELD-EFFECT TRANSISTOR ; LIGHT ; CHANNELRHODOPSIN-2 ; FABRICATION ; ASSAYS ; FILMS |
WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
语种 | 英语 |
WOS记录号 | WOS:000783050700006 |
资助机构 | National Key R&D Program of China (Grant no. 2018YFB1304700) ; National Natural Science Foundation of China (Grant nos. U1908215, 61903157, 91848201, 61973316) ; Liaoning Revitalization Talents Program (Grant no. XLYC2002014) |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/30524] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Wang WX(王文学) |
作者单位 | 1.School of Electromechanical and Automotive Engineering, Yantai University, Yantai 264005, China 2.Department of Biochemistry & Molecular Biology, China Medical University, Shenyang 110122, China 3.Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, China 4.Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), Institute of Automation, Jiangnan University, Wuxi 214122, China 5.University of Chinese Academy of Sciences, Beijing 100049, China 6.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110169, China 7.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China |
推荐引用方式 GB/T 7714 | Yang J,Li GX,Zu LP,et al. Optogenetically engineered cell-based graphene transistor for pharmacodynamic evaluation of anticancer drugs[J]. Sensors and Actuators B: Chemical,2022,358:1-11. |
APA | Yang J.,Li GX.,Zu LP.,Wang WX.,Ge ZX.,...&Liu LQ.(2022).Optogenetically engineered cell-based graphene transistor for pharmacodynamic evaluation of anticancer drugs.Sensors and Actuators B: Chemical,358,1-11. |
MLA | Yang J,et al."Optogenetically engineered cell-based graphene transistor for pharmacodynamic evaluation of anticancer drugs".Sensors and Actuators B: Chemical 358(2022):1-11. |
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