Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling | |
Zare-Eelanjegh, Elaheh1; Luchtefeld, Ines1; Li M(李密)3; Sarajlic, Edin2; Zambelli, Tomaso1 | |
刊名 | EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS |
2021 | |
卷号 | 50期号:S1页码:202-202 |
ISSN号 | 0175-7571 |
产权排序 | 2 |
英文摘要 | Understanding the interactions between biochemical and mechanical cues in cellular behaviors is a key for revealing numerous biological functions and development of major diseases. This necessitates creating tools enabling in situ monitoring and quantifying such small forces in intact cells. Mechanosensory complexes at the cellular level are such a demanding situation in which the dynamic interconnectivity of the cytoskeleton, nuclear envelope, and nucleoskeleton is yet to be understood. Fluidic force microscopy (FluidFM), a force-controlled micropipette configured on an atomic force microscope (AFM) enables to manipulate living cells while delivering non-permeable sensory biomolecules into their cytosol (or nucleus) for parallel screening of molecular responses. This will be achieved owing to the versatility of FluidFM tips and gentleness of cantilever-membrane contact. Thereby, I will elaborate FluidFM combined with sophisticated time-resolved optical readouts such as fluorescencelifetime imaging microscopy (FLIM) and fluorescence resonance energy transfer (FRET)-based systems to address the dynamic mechanosensory of essential components of the linker of nucleoskeleton and cytoskeleton (LINC) complex. |
WOS研究方向 | Biophysics |
语种 | 英语 |
WOS记录号 | WOS:000671622300564 |
内容类型 | 期刊论文 |
源URL | [http://ir.sia.cn/handle/173321/29415] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Zare-Eelanjegh, Elaheh |
作者单位 | 1.Laboratory for Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Switzerland 2.MESA+ Institute for Nanotechnology, University of Twente, Netherlands 3.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, China |
推荐引用方式 GB/T 7714 | Zare-Eelanjegh, Elaheh,Luchtefeld, Ines,Li M,et al. Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling[J]. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,2021,50(S1):202-202. |
APA | Zare-Eelanjegh, Elaheh,Luchtefeld, Ines,Li M,Sarajlic, Edin,&Zambelli, Tomaso.(2021).Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling.EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS,50(S1),202-202. |
MLA | Zare-Eelanjegh, Elaheh,et al."Fluidic force microscopy for monitoring and quantifying the nucleo-cytoskeletal coupling".EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS 50.S1(2021):202-202. |
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