Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)

doi:10.1016/j.ecoenv.2021.112994

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生态与环境科学重点实验室

	Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)
	Huo, Da 2,4,5,6,7; Sun, Lina 2,3,4,5,6,7; Sun, Jingchun 2,4,5,6,7; Lin, Chenggang 2,4,5,6,7; Liu, Shilin 2,4,5,6,7; Zhang, Libin 2,3,4,5,6,7; Yang, Hongsheng 1,2,3,4,5,6,7
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2021-12-25
卷号	228 页码:9
关键词	Circular RNA Echinoderm Heat Anoxia Environmental stress
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2021.112994
通讯作者	Sun, Lina(sunlina@qdio.ac.cn)
英文摘要	Some sea cucumbers are economically and ecologically important, but they are threatened by thermal and hypoxic stress in changing oceanographic conditions. We construct circRNAs profiles, reveal circRNAs characters, and illustrate the potential regulatory roles of circRNAs in one commercially important species of sea cucumber, Apostichopus japonicus. Reads are distributed in intergenic (44.14%), exonic (48.26%) and intronic (7.60%) regions of the genome. A total of 1684 circRNAs were identified, and the most common spliced length is 269 nt in the present study. In three treatments (HT [thermal stress], LO [hypoxic stress], and HL [combined thermal and hypoxic stress]), 24, 27 and 27 differentially expressed (DE) circRNAs were identified, respectively. Five novel DE-circRNAs commonly occur in these treatments (novel_circ_0003311, novel_circ_0000229, novel_circ_0003944, novel_circ_0001458 and novel_circ_0000707), and based on them, potential circRNA-miRNA binding pairs were predicted. Sanger sequencing, RNase R treatment experiment and qPCR validation identified the accuracy of the circRNAs. Key circRNAs identified in the present study were covalently closed and were more stable under RNase R treatment than linear RNAs. Based on function analysis, circRNAs could regulate metabolic process, signal transduction, and ion responses in A. japonicus when exposed to thermal and hypoxic stress, and 'regulation of response to stimulus' is a common gene ontology (GO) term that is significantly enriched in each treatment; GO terms for 'DNA' and 'stress' are commonly enriched in heat-related treatments (HT and HL); and GO terms for 'protein' are commonly enriched in hypoxia-related treatments (LO and HL). When environmentally stressed, 'metabolism,' 'transport and catabolism,' 'membrane transport,' and 'signal transduction' were significantly responded in sea cucumber based on KEGG analysis. We provide insights into circRNA functions in stress regulation and lay a foundation for invertebrate circRNA research.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA24030304] ; National Natural Science Foundation of China[42106106] ; National Natural Science Foundation of China[42030408] ; China Postdoctoral Science Foundation[2020M682247] ; Postdoctoral Innovative Talents Support Program of Shandong Province[SDBX2020006] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010812] ; Shandong Provincial Natural Science Foundation[ZR202102200540] ; Special Research Assistant Project of Chinese Academy of Sciences, Qingdao Postdoctoral Applied Research Project[2020170] ; Youth Innovation Promotion Association CAS[2019209]
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000744241900006
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177910]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Sun, Lina
作者单位	1.Chinese Acad Sci, Innovat Seed Design, Wuhan 430071, Peoples R China 2.Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Engn Lab Marine Ranching, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 6.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Huo, Da,Sun, Lina,Sun, Jingchun,et al. Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,228:9.
APA	Huo, Da.,Sun, Lina.,Sun, Jingchun.,Lin, Chenggang.,Liu, Shilin.,...&Yang, Hongsheng.(2021).Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea).ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,228,9.
MLA	Huo, Da,et al."Emerging roles of circRNAs in regulating thermal and hypoxic stresses in Apostichopus japonicus (Echinodermata: Holothuroidea)".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 228(2021):9.