Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy

doi:10.1080/13510002.2022.2036507

	Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy
	Zhu, Sen 1; Li, Xuan 2; Dang, Bingrong 3; Wu, Fen 1; Wang, Chunming 1; Lin, Changjun 1
刊名	REDOX REPORT
	2022-12-31
卷号	27 期号:1 页码:32-44
关键词	PM2 5 Lycium barbarum polysaccharide (LBP) antioxidant oxidative damage endoplasmic reticulum (ER) stress autophagy apoptosis mitochondrial damage
ISSN号	1351-0002
DOI	10.1080/13510002.2022.2036507
通讯作者	Lin, Changjun(linc@lzu.edu.cn)
英文摘要	Objectives: Lycium barbarum polysaccharide (LBP) is a natural polysaccharide extracted from Lycium barbarum that has anti-inflammatory, anti-apoptotic and anti-aging effects, and plays a role in the prevention and treatment of various diseases. In this study, we investigated the therapeutic effect of LBP on particulate matter 2.5 (PM2.5)-induced skin damage. Methods: Cell viability was analyzed by MTT and LDH assays. Apoptosis was analyzed by Annexin V-FITC/PI staining. Oxidative stress/damage were assessed by intracellular ROS levels, MDA content and SOD activity. The intracellular protein expression was analyzed by Western blot. Mitochondrial damage was assayed by mitochondrial membrane potential with JC-1 probe. LC3-GFP adenovirus was transfected into HaCaT cells to analyze intracellular autophagosome levels. Results: In PM2.5-treated HaCaT cells, LBP pretreatment reduced PM2.5-induced cytotoxicity, ameliorated cell morphology and reduced cell apoptosis. LBP also inhibited the expression levels of GRP78 and CHOP, reduced the conversion of LC3I to LC3II, inhibited Bax protein and activated Bcl-2 protein. Furthermore, LBP inhibited PM2.5-induced mitochondrial autophagy (mitophagy) and mitochondrial damage. PM2.5-induced autophagy was regulated by endoplasmic reticulum (ER) stress. Conclusion: LBP protects skin cells from PM2.5-induced cytotoxicity by regulating the oxidative stress-ER stress-autophagy-apoptosis signaling axis, revealing that LBP has a great potential for the skin protection.
资助项目	LZU-IMPCAS cooperation
WOS关键词	KERATINOCYTES ; CANCER ; DAMAGE
WOS研究方向	Biochemistry & Molecular Biology
语种	英语
出版者	TAYLOR & FRANCIS LTD
WOS记录号	WOS:000752638300001
资助机构	LZU-IMPCAS cooperation
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/142135]
专题	中国科学院近代物理研究所
通讯作者	Lin, Changjun
作者单位	1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Lanzhou Univ Second Hosp, Lanzhou, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Sen,Li, Xuan,Dang, Bingrong,et al. Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy[J]. REDOX REPORT,2022,27(1):32-44.
APA	Zhu, Sen,Li, Xuan,Dang, Bingrong,Wu, Fen,Wang, Chunming,&Lin, Changjun.(2022).Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy.REDOX REPORT,27(1),32-44.
MLA	Zhu, Sen,et al."Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy".REDOX REPORT 27.1(2022):32-44.