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	Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy
	Zhu, Sen1; Li, Xuan2; Dang, Bingrong3; Wu, Fen1; Wang, Chunming1; Lin, Changjun1
Source	REDOX REPORT
	2022-12-31
Volume	27Issue:1Pages:32-44
Keyword	PM2 5 Lycium barbarum polysaccharide (LBP) antioxidant oxidative damage endoplasmic reticulum (ER) stress autophagy apoptosis mitochondrial damage
ISSN	1351-0002
DOI	10.1080/13510002.2022.2036507
Corresponding Author	Lin, Changjun(linc@lzu.edu.cn)
English Abstract	Objectives: Lycium barbarum polysaccharide (LBP) is a natural polysaccharide extracted from Lycium barbarum that has anti-inflammatory, anti-apoptotic and anti-aging effects, and plays a role in the prevention and treatment of various diseases. In this study, we investigated the therapeutic effect of LBP on particulate matter 2.5 (PM2.5)-induced skin damage. Methods: Cell viability was analyzed by MTT and LDH assays. Apoptosis was analyzed by Annexin V-FITC/PI staining. Oxidative stress/damage were assessed by intracellular ROS levels, MDA content and SOD activity. The intracellular protein expression was analyzed by Western blot. Mitochondrial damage was assayed by mitochondrial membrane potential with JC-1 probe. LC3-GFP adenovirus was transfected into HaCaT cells to analyze intracellular autophagosome levels. Results: In PM2.5-treated HaCaT cells, LBP pretreatment reduced PM2.5-induced cytotoxicity, ameliorated cell morphology and reduced cell apoptosis. LBP also inhibited the expression levels of GRP78 and CHOP, reduced the conversion of LC3I to LC3II, inhibited Bax protein and activated Bcl-2 protein. Furthermore, LBP inhibited PM2.5-induced mitochondrial autophagy (mitophagy) and mitochondrial damage. PM2.5-induced autophagy was regulated by endoplasmic reticulum (ER) stress. Conclusion: LBP protects skin cells from PM2.5-induced cytotoxicity by regulating the oxidative stress-ER stress-autophagy-apoptosis signaling axis, revealing that LBP has a great potential for the skin protection.
Funding Project	LZU-IMPCAS cooperation
WOS Keyword	KERATINOCYTES ; CANCER ; DAMAGE
WOS Research Area	Biochemistry & Molecular Biology
Language	英语
Publisher	TAYLOR & FRANCIS LTD
WOS ID	WOS:000752638300001
Funding Organization	LZU-IMPCAS cooperation
Content Type	期刊论文
源URL	[http://119.78.100.186/handle/113462/142135]
Collection	中国科学院近代物理研究所
Corresponding Author	Lin, Changjun
Affiliation	1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China 2.Lanzhou Univ Second Hosp, Lanzhou, Peoples R China 3.Chinese Acad Sci, Inst Modern Phys, Lanzhou, Peoples R China
Recommended Citation GB/T 7714	Zhu, Sen,Li, Xuan,Dang, Bingrong,et al. Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy[J]. REDOX REPORT,2022,27(1):32-44.
APA	Zhu, Sen,Li, Xuan,Dang, Bingrong,Wu, Fen,Wang, Chunming,&Lin, Changjun.(2022).Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy.REDOX REPORT,27(1),32-44.
MLA	Zhu, Sen,et al."Lycium Barbarum polysaccharide protects HaCaT cells from PM2.5-induced apoptosis via inhibiting oxidative stress, ER stress and autophagy".REDOX REPORT 27.1(2022):32-44.

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