An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity

doi:10.1016/j.carbpol.2021.118430

	An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity
	Su, Ziran 1; Luo, Jianquan 2; Sigurdardottir, Sigyn Bjork 1; Manferrari, Thomas 1; Jankowska, Katarzyna 1,3; Pinelo, Manuel 1
刊名	CARBOHYDRATE POLYMERS
	2021-11-01
卷号	271 页码:10
关键词	Enzymatic membrane reactor Enzyme immobilization Dextranase Oligodextran Biocatalytic membrane
ISSN号	0144-8617
DOI	10.1016/j.carbpol.2021.118430
英文摘要	An enzymatic membrane reactor (EMR) with immobilized dextranase provides an excellent opportunity for tailoring the molecular weight (Mw) of oligodextran to significantly improve product quality. However, a highly efficient EMR for oligodextran production is still lacking and the effect of enzyme immobilization strategy on dextranase hydrolysis behavior has not been studied yet. In this work, a functional layer of polydopamine (PDA) or nanoparticles made of tannic acid (TA) and hydrolysable 3-amino-propyltriethoxysilane (APTES) was first coated on commercial membranes. Then cross-linked dextranase or non-cross-linked dextranase was loaded onto the modified membranes using incubation mode or fouling-induced mode. The fouling-induced mode was a promising enzyme immobilization strategy on the membrane surface due to its higher enzyme loading and activity. Moreover, unlike the non-cross-linked dextranase that exhibited a normal endo-hydrolysis pattern, we surprisingly found that the cross-linked dextranase loaded on the PDA modified surface exerted an exo-hydrolysis pattern, possibly due to mass transfer limitations. Such alteration of hydrolysis pattern has rarely been reported before. Based on the hydrolysis behavior of the immobilized dextranase in different EMRs, we propose potential applications for the oligodextran products. This study presents a unique perspective on the relation between the enzyme immobilization process and the immobilized enzyme hydrolysis behavior, and thus opens up a variety of possibilities for the design of a high-performance EMR.
资助项目	Novo Nordisk Fonden[NNF19OC0057684] ; China Scholarship Council[201804910747] ; Poznan University of Technology, Poland
WOS关键词	INTERFACIAL INTERACTIONS ; SURFACE ; GLUTARALDEHYDE ; CHALLENGES ; FILTRATION ; STABILITY ; BEHAVIOR ; DESIGN ; IMPACT ; CLEAS
WOS研究方向	Chemistry ; Polymer Science
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000685091700006
资助机构	Novo Nordisk Fonden ; China Scholarship Council ; Poznan University of Technology, Poland
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49907]
专题	中国科学院过程工程研究所
通讯作者	Luo, Jianquan; Pinelo, Manuel
作者单位	1.Tech Univ Denmark, Proc & Syst Engn Ctr, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark 2.Chinese Acad Sci, Univ Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Poznan Univ Tech, Fac Chem Technol, Inst Chem Technol & Engn, Berdychowo 4, PL-60965 Poznan, Poland
推荐引用方式 GB/T 7714	Su, Ziran,Luo, Jianquan,Sigurdardottir, Sigyn Bjork,et al. An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity[J]. CARBOHYDRATE POLYMERS,2021,271:10.
APA	Su, Ziran,Luo, Jianquan,Sigurdardottir, Sigyn Bjork,Manferrari, Thomas,Jankowska, Katarzyna,&Pinelo, Manuel.(2021).An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity.CARBOHYDRATE POLYMERS,271,10.
MLA	Su, Ziran,et al."An enzymatic membrane reactor for oligodextran production: Effects of enzyme immobilization strategies on dextranase activity".CARBOHYDRATE POLYMERS 271(2021):10.