Reduction of choroidal neovascularization via cleavable VEGF antibodies conjugated to exosomes derived from regulatory T cells

doi:10.1038/s41551-021-00764-3

	Reduction of choroidal neovascularization via cleavable VEGF antibodies conjugated to exosomes derived from regulatory T cells
	Tian, Ying 1,2; Zhang, Fan 2,3; Qiu, Yefeng 4; Wang, Shuang 2; Li, Feng 2,5; Zhao, Jiawei 2; Pan, Chao 6; Tao, Yong 1; Yu, Di 7; Wei, Wei 2,5
刊名	NATURE BIOMEDICAL ENGINEERING
	2021-07-26
页码	19
ISSN号	2157-846X
DOI	10.1038/s41551-021-00764-3
英文摘要	Intravitreally injected exosomes derived from regulatory T cells and conjugated with an antibody for vascular endothelial growth factor via a cleavable linker markedly suppress ocular neovascularization in mice and non-human primates. Choroidal neovascularization induced by age-related macular degeneration and retinal neovascularization induced by diabetic retinopathy-two leading causes of blindness-are often treated using antibodies targeting vascular endothelial growth factor (VEGF). Here we report a strong association between inflammation and high VEGF expression in aqueous humour samples from patients with choroidal or retinal neovascularization, and show that intravitreally injected exosomes derived from regulatory T cells and conjugated with an anti-VEGF antibody via a peptide linker that is cleavable by matrix metalloproteinases markedly suppressed ocular neovascularization in mouse and non-human primate models of choroidal neovascularization. The engineered exosomes, which selectively accumulate in the neovascularization lesions, could be adapted for other combination therapies of therapeutic antibodies and anti-inflammatory cargo.
资助项目	National Natural Science Foundation of China[82070948] ; National Natural Science Foundation of China[U2001224] ; Beijing Chaoyang 1351 talent training programme[CYXX-2017-21] ; Scientific Research Program of Beijing Municipal Commission of Education[KM202010025020]
WOS关键词	MACULAR DEGENERATION ; INFLAMMATORY CYTOKINES ; INTRAVITREAL DEXAMETHASONE ; AQUEOUS-HUMOR ; BEVACIZUMAB ; RANIBIZUMAB ; MODELS ; NAIVE
WOS研究方向	Engineering
语种	英语
出版者	NATURE RESEARCH
WOS记录号	WOS:000677707300002
资助机构	National Natural Science Foundation of China ; Beijing Chaoyang 1351 talent training programme ; Scientific Research Program of Beijing Municipal Commission of Education
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49522]
专题	中国科学院过程工程研究所
通讯作者	Tao, Yong; Yu, Di; Wei, Wei
作者单位	1.Capital Med Univ, Beijing Chaoyang Hosp, Dept Ophthalmol, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 3.Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Shenzhen, Peoples R China 4.Acad Mil Med Sci, Anim Ctr, Beijing, Peoples R China 5.Univ Chinese Acad Sci, Sch Chem Engn, Beijing, Peoples R China 6.Beijing Inst Biotechnol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China 7.Univ Queensland, Fac Med, Diamantina Inst, Brisbane, Qld, Australia
推荐引用方式 GB/T 7714	Tian, Ying,Zhang, Fan,Qiu, Yefeng,et al. Reduction of choroidal neovascularization via cleavable VEGF antibodies conjugated to exosomes derived from regulatory T cells[J]. NATURE BIOMEDICAL ENGINEERING,2021:19.
APA	Tian, Ying.,Zhang, Fan.,Qiu, Yefeng.,Wang, Shuang.,Li, Feng.,...&Wei, Wei.(2021).Reduction of choroidal neovascularization via cleavable VEGF antibodies conjugated to exosomes derived from regulatory T cells.NATURE BIOMEDICAL ENGINEERING,19.
MLA	Tian, Ying,et al."Reduction of choroidal neovascularization via cleavable VEGF antibodies conjugated to exosomes derived from regulatory T cells".NATURE BIOMEDICAL ENGINEERING (2021):19.