Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system

doi:10.1002/elsc.202000091

	Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system
	Wang, Xu-Dong 1,2; Hu, Chun-Yan 1; Qin, Chao 1; Dong, Yue-Sheng 1; Ying, Guo-Qing 2; Xiu, Zhi-Long 1; Su, Zhi-Guo 3
刊名	ENGINEERING IN LIFE SCIENCES
	2021-07-29
页码	10
关键词	clavulanic acid fermentation broth salting-out extraction solid-liquid separation
ISSN号	1618-0240
DOI	10.1002/elsc.202000091
英文摘要	Clavulanic acid (CA) is usually used together with other beta-lactam antibiotics as combination drugs to inhibit bacterial beta-lactamases, which is mainly produced from the fermentation of microorganism such as Streptomyces clavuligerus. Recently, it is still a challenge for downstream processing of low concentration and unstable CA from fermentation broth with high solid content, high viscosity, and small cell size. In this study, an integrated process was developed for simultaneous solid-liquid separation and primary purification of CA from real fermentation broth of S. clavuligerus using salting-out extraction system (SOES). First, different SOESs were investigated, and a suitable SOES composed of ethanol/phosphate was chosen and further optimized using the pretreated fermentation broth. Then, the optimal system composed of 20% ethanol/15% K2HPO4 and 10% KH2PO4 w/w was used to direct separation of CA from untreated fermentation broth. The result showed that the partition coefficient (K) and recovery yield (Y) of CA from untreated fermentation broth were 29.13 and 96.8%, respectively. Simultaneously, the removal rates of the cells and proteins were 99.8% and 63.3%, respectively. Compared with the traditional method of membrane filtration or liquid-liquid extraction system, this developed SOES showed the advantages of simple operation, shorter operation time, lower process cost and higher recovery yield of CA. These results demonstrated that the developed SOES could be used as an attractive alternative for the downstream processing of CA from real fermentation broth.
资助项目	National Natural Science Foundation of China[22078042] ; Natural Science Foundation of Zhejiang Province[LY21B060007]
WOS关键词	AQUEOUS 2-PHASE SYSTEM ; STABILITY ; 1,3-PROPANEDIOL ; 2,3-BUTANEDIOL
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	WILEY
WOS记录号	WOS:000679128800001
资助机构	National Natural Science Foundation of China ; Natural Science Foundation of Zhejiang Province
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49516]
专题	中国科学院过程工程研究所
通讯作者	Xiu, Zhi-Long
作者单位	1.Dalian Univ Technol, Sch Bioengn, 2 Linggong Rd, Dalian 116024, Peoples R China 2.Zhejiang Univ Technol, Coll Pharmaceut Sci, Hangzhou, Zhejiang, Peoples R China 3.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xu-Dong,Hu, Chun-Yan,Qin, Chao,et al. Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system[J]. ENGINEERING IN LIFE SCIENCES,2021:10.
APA	Wang, Xu-Dong.,Hu, Chun-Yan.,Qin, Chao.,Dong, Yue-Sheng.,Ying, Guo-Qing.,...&Su, Zhi-Guo.(2021).Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system.ENGINEERING IN LIFE SCIENCES,10.
MLA	Wang, Xu-Dong,et al."Simultaneous solid-liquid separation and primary purification of clavulanic acid from fermentation broth of Streptomyces clavuligerus using salting-out extraction system".ENGINEERING IN LIFE SCIENCES (2021):10.