Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens

doi:10.1021/acsami.1c05522

	Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens
	Hu, Qiushi 1,2; Wu, Qixiao 1; Huang, Fengchun 2; Xu, Zheng 1; Zhou, Lei 2; Zhao, Suling 1
刊名	ACS APPLIED MATERIALS & INTERFACES
	2021-06-16
卷号	13 期号:23 页码:26782-26789
关键词	multicolor coding up-conversion nanoparticles photoluminescence multiplex detection foodborne pathogen
ISSN号	1944-8244
DOI	10.1021/acsami.1c05522
英文摘要	Technologies for rapid screening of multiple foodborne pathogens have been urgently needed because of the complex food matrix and high outbreaks of foodborne diseases. In this study, multicolor coding up-conversion nanoparticles (UCNPs) were synthesized and applied for rapid and simultaneous detection of five kinds of foodborne pathogens. The multicolor coding UCNPs were obtained through doping different concentrations of a sensitizer (Yb3+) on the shell of the synthesized NaYF4:Yb3+, Tm3+ (20%/2%)@NaYF4:Yb3+, and Er3+ (x %/2%) core/shell nanocrystals. All the UCNPs could emit red and green luminescence simultaneously once excited with near-infrared wavelength (980 nm), and the ratio of red and green (R/G ratio) emission light intensity of each kind of UCNPs varied depending on the Yb3+ doping concentration. In addition, the magnetic nanoparticles (MNPs) modified with the monoclonal antibodies (mAbs) against the target bacteria were used to capture and separate the bacteria, resulting in obtaining the MNP-bacterium complexes. Different UCNPs with multicolor coding acted as signal probes were also modified with the mAbs to react with the MNP-bacterium complexes to form the MNP-bacterium-UCNP sandwich complexes. After the sandwich complexes were excited with a wavelength of 980 nm, the obtained R/G ratios and the green photoluminescence intensity (PL intensity) could be used to distinguish and quantitatively detect foodborne pathogens, respectively. This proposed nanoplatform could detect five foodborne pathogens simultaneously within 2 h with good sensitivity and specificity, showing great potential for multiplex detection of other targets in the fields of medical diagnosis and food security.
资助项目	National Key Research and Development Program[2019YFC1606600] ; National Key Research and Development Program[2019YFC1606602] ; Beijing Nova Programme Interdisciplinary Cooperation Project[Z191100001119005] ; Independent Research Project of State Key Laboratory of Biochemical Engineering - Innovation Academy for Green Manufacture, Chinese Academy of Sciences[2020ZZ-03] ; Independent Research Project of State Key Laboratory of Biochemical Engineering - Innovation Academy for Green Manufacture, Chinese Academy of Sciences[IAGM2020C31] ; [BWS18C015] ; [18-163-12-ZT-005-016-01] ; [19-H863-18-ZD-011-004-03] ; [17SAZ08] ; [2019-JCJQ-ZD-117-05-1] ; [20-163-12-ZT-006-003-02]
WOS关键词	QUANTITATIVE DETECTION ; SALMONELLA ; NANOCRYSTALS ; BACTERIA ; ASSAY ; PCR ; RED ; LUMINESCENCE ; MICROARRAYS ; EMISSION
WOS研究方向	Science & Technology - Other Topics ; Materials Science
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000664289800012
资助机构	National Key Research and Development Program ; Beijing Nova Programme Interdisciplinary Cooperation Project ; Independent Research Project of State Key Laboratory of Biochemical Engineering - Innovation Academy for Green Manufacture, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49288]
专题	中国科学院过程工程研究所
通讯作者	Zhou, Lei; Zhao, Suling
作者单位	1.Beijing Jiaotong Univ, Inst Optoelect Technol, Key Lab Luminescence & Opt Informat, Minist Educ, Beijing 100044, Peoples R China 2.Chinese Acad Sci, Natl Key Lab Biochem Engn, PLA Key Lab Biopharmaceut Prod & Formulat Engn, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Hu, Qiushi,Wu, Qixiao,Huang, Fengchun,et al. Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens[J]. ACS APPLIED MATERIALS & INTERFACES,2021,13(23):26782-26789.
APA	Hu, Qiushi,Wu, Qixiao,Huang, Fengchun,Xu, Zheng,Zhou, Lei,&Zhao, Suling.(2021).Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens.ACS APPLIED MATERIALS & INTERFACES,13(23),26782-26789.
MLA	Hu, Qiushi,et al."Multicolor Coding Up-Conversion Nanoplatform for Rapid Screening of Multiple Foodborne Pathogens".ACS APPLIED MATERIALS & INTERFACES 13.23(2021):26782-26789.