Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants

doi:10.1016/j.cej.2020.127870

	Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants
	Zhang, Hao 2; Luo, Jianquan 2; Woodley, John M.1; Wan, Yinhua 2
刊名	CHEMICAL ENGINEERING JOURNAL
	2021-10-01
卷号	421 页码:12
关键词	Micropollutants Biocatalytic membrane Enzyme mobility Enzyme immobilization Confinement strength
ISSN号	1385-8947
DOI	10.1016/j.cej.2020.127870
英文摘要	Enzymes in living cells are highly dynamic but at the same time regularly confined for achieving efficient metabolism. Inspired by this phenomenon, we have prepared a novel biocatalytic membrane with high enzyme activity and stability by tuning the confinement strength of the membrane to enzyme, which was achieved via modifying the support layer of a polymeric nanofiltration (NF) membrane and reversely filtrating enzyme. A mussel-inspired coating was used to modify the support interior of the NF membrane to enhance charge and steric effects on enzyme, thus stabilizing enzyme in the membrane with little increment in mass transfer resistance for substrate and products (only 20% permeability loss with a high enzyme loading of 1.34 mg/cm2). A suitable confinement strength of the membrane to enzyme could delay the enzyme leakage and endow enzyme with certain mobility for efficient reaction. Thus, the obtained biocatalytic membrane exhibited a negligible decline in BPA removal efficiency for 7 reuse cycles (<3.5%) or 36 h continuous operation (<1%) in flow through mode, resulting in a long-term stability adequate for micropollutant removal. For the first time, enzyme mobility was defined and calculated to quantify the confinement strength of the membrane, which could be used to optimize the microenvironment for enzyme immobilization and predict the performance of the biocatalytic membrane. This work concluded that rationally regulating the enzyme mobility in the membrane and a periodic back-flushing operation for redistribution of enzymes could achieve a long-term stable removal of micropollutant in water by a biocatalytic membrane.
资助项目	National Natural Science Foundation of China[21878306] ; Beijing Natural Science Foundation[2192053]
WOS关键词	ORGANIC MICROPOLLUTANTS ; BISPHENOL-A ; IMMOBILIZATION ; LACCASE ; PERFORMANCE ; STABILITY
WOS研究方向	Engineering
语种	英语
出版者	ELSEVIER SCIENCE SA
WOS记录号	WOS:000663713700005
资助机构	National Natural Science Foundation of China ; Beijing Natural Science Foundation
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/49235]
专题	中国科学院过程工程研究所
通讯作者	Luo, Jianquan
作者单位	1.Tech Univ Denmark, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark 2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Hao,Luo, Jianquan,Woodley, John M.,et al. Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants[J]. CHEMICAL ENGINEERING JOURNAL,2021,421:12.
APA	Zhang, Hao,Luo, Jianquan,Woodley, John M.,&Wan, Yinhua.(2021).Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants.CHEMICAL ENGINEERING JOURNAL,421,12.
MLA	Zhang, Hao,et al."Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants".CHEMICAL ENGINEERING JOURNAL 421(2021):12.