Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii

doi:10.1007/s12010-021-03584-y

	Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii
	Zhang, Yuhan 1,2; Li, Xiaolian 2; Wang, Ziqiang 2; Wang, Yunshan 2; Ma, Yuanyuan 1; Su, Zhiguo 2
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2021-05-15
页码	17
关键词	Propionibacterium freudenreichii Vitamin B-12 Propionic acid Membrane separation coupled fermentation Metabolic flux analysis
ISSN号	0273-2289
DOI	10.1007/s12010-021-03584-y
英文摘要	The metabolic processes involved in simultaneous production of vitamin B-12 and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanism of this metabolism, a simplified metabolic network was established. The effects of glucose feeding, propionic acid removal, and 5,6-dimethylbenzimidazole (DMB) addition on the metabolic flux distribution were investigated. The results showed that synthesis of propionic acid can be increased by increasing the metabolic flux through the oxaloacetate and methylmalonyl-CoA branches in the early and middle stages of the coupled fermentation. After DMB addition, the synthesis of vitamin B-12 was significantly enhanced via increased metabolic flux through the delta-aminolevulinate branch, which promoted the synthesis of uroporphyrinogen III, a precursor of vitamin B-12. Therefore, the analysis of metabolic flux at key nodes can provide theoretical guidance for the optimization of P. freudenreichii fermentation processes. In an experimental coupled fermentation process, the concentrations of vitamin B-12 and propionic acid reached 21.6 and 50.12 g/L respectively, increased by 105.71% and 73.91% compared with batch fermentation, which provides a new strategy for industrial production.
资助项目	National Natural Science Foundation of China[21506227]
WOS关键词	MICROBIAL-PRODUCTION ; COBALAMIN VITAMIN-B-12 ; IMMOBILIZED CELLS ; ESCHERICHIA-COLI ; BIOSYNTHESIS ; ACIDIPROPIONICI ; GLYCEROL ; SHERMANII ; BATCH
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000650521200001
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/48704]
专题	中国科学院过程工程研究所
通讯作者	Wang, Ziqiang; Ma, Yuanyuan
作者单位	1.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yuhan,Li, Xiaolian,Wang, Ziqiang,et al. Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2021:17.
APA	Zhang, Yuhan,Li, Xiaolian,Wang, Ziqiang,Wang, Yunshan,Ma, Yuanyuan,&Su, Zhiguo.(2021).Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,17.
MLA	Zhang, Yuhan,et al."Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2021):17.