Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus

doi:10.1016/j.biortech.2020.124018

	Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus
	Sharshar, Moustafa Mohamed 3,4; Samak, Nadia Abdrabou 3,4,5; Ambreen, Sadaf 6; Hao, Xuemi 3,4; Mu, Tingzhen 3; Maarouf, Mohamed 1,7; Zheng, Chen 3,4; Gao, Yibo 3,4; Liu, Zhixia 3; Jia, Yunpu 3,4
刊名	BIORESOURCE TECHNOLOGY
	2020-12-01
卷号	317 页码:11
关键词	Nanometric sulfur bioproduction Desulfurization Cell death-induced CRISPR Sulfur pathways Thioalkalivibrio versutus
ISSN号	0960-8524
DOI	10.1016/j.biortech.2020.124018
英文摘要	Complicated production procedures and superior characteristics of nano-sized sulfur elevate its price to 25-40 fold higher than micrograde kind. Also, natural gas hydrogen sulfide levels are restricted because of its toxic environmental consequences. Thioalkalivibrio versutus is a polyextremophilic industrial autotroph with high natural gas desulfurization capability. Here, nanometric ( > 50 nm) sulfur bioproduction using T. versutus while desulfurizing natural gas was validated. Also, this production was enhanced by 166.7% via lowering sulfate production by 55.1%. A specially-developed CRISPR system, with 42% editing efficiency, simplified the genome editing workflow scheme for this challenging bacterium. In parallel, sulfur metabolism was uncovered using proteins mining and transcriptome studies for defining sulfate-producing key genes (heterodisulfide reductase-like complex, sulfur dioxygenase, sulfite dehydrogenase and sulfite oxidase). This study provided cost-effective nanometric sulfur production and improved this production using a novel CRISPR strategy, which could be suitable for industrial polyextremophiles, after uncovering sulfur pathways in T. versutus.
资助项目	National Science Foundation of China[21878307] ; National Science Foundation of China[31872633] ; National Science Foundation of China[31800030] ; National Key R&D Program of China[2020YFA0906800] ; CAS-TWAS (The World Academy of Sciences)
WOS关键词	FED-BATCH ; GENOME ; PERFORMANCE ; CRISPR ; OXIDATION ; SEQUENCE ; PLATFORM ; PCR
WOS研究方向	Agriculture ; Biotechnology & Applied Microbiology ; Energy & Fuels
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000577077400008
资助机构	National Science Foundation of China ; National Key R&D Program of China ; CAS-TWAS (The World Academy of Sciences)
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/42409]
专题	中国科学院过程工程研究所
通讯作者	Xing, Jianmin
作者单位	1.Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Guangdong Prov Key Lab Synthet Genom, Shenzhen Key Lab Synthet Genom,Shenzhen Inst Adv, CAS Key Lab Quantitat Engn Biol,Shenzhen Inst Syn, Shenzhen 518055, Peoples R China 3.Chinese Acad Sci, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Inst Proc Engn, Beijing 100190, Peoples R China 4.Univ Chinese Acad Sci, Coll Chem Engn, 19 A Yuquan Rd, Beijing 100049, Peoples R China 5.Egyptian Petr Res Inst, Proc Design & Dev Dept, Cairo 11727, Egypt 6.Chinese Acad Sci, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China 7.Suez Canal Univ, Fac Vet Med, Dept Virol, Ismailia 41522, Egypt
推荐引用方式 GB/T 7714	Sharshar, Moustafa Mohamed,Samak, Nadia Abdrabou,Ambreen, Sadaf,et al. Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus[J]. BIORESOURCE TECHNOLOGY,2020,317:11.
APA	Sharshar, Moustafa Mohamed.,Samak, Nadia Abdrabou.,Ambreen, Sadaf.,Hao, Xuemi.,Mu, Tingzhen.,...&Xing, Jianmin.(2020).Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus.BIORESOURCE TECHNOLOGY,317,11.
MLA	Sharshar, Moustafa Mohamed,et al."Improving confirmed nanometric sulfur bioproduction using engineered Thioalkalivibrio versutus".BIORESOURCE TECHNOLOGY 317(2020):11.